| Description |
1 online resource (xiv, 298 pages) : illustrations |
| Contents |
Cover -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction -- 1.1 Introduction -- 1.2 Centrifugal Separation and Filtration -- 1.2.1 Sedimenting Centrifuge -- 1.2.2 Filtering Centrifuges -- 1.3 Pros and Cons of Filtration versus Centrifugation -- 1.4 Generic Flow Sheet for Biopharmaceutical Process -- 1.5 Other Centrifugal Separations -- 1.6 Inputs and Outputs of Centrifuge -- 1.7 Separation Metrics -- 1.7.1 Protein Yield -- 1.7.2 Centrate Suspended Solids -- 1.7.3 Throughput Rate -- 1.7.4 Cell Viability -- 1.8 Summary -- References -- Problems -- Chapter 2 Principles of Centrifugal Sedimentation -- 2.1 Introduction -- 2.2 Non-intuitive Phenomena -- 2.2.1 Pressure Distribution -- 2.2.2 Coriolis Effect -- 2.3 Intuitive Phenomena -- 2.3.1 Centrifugal Acceleration -- 2.3.2 Fluid in a Centrifuge Bowl not at Solid-Body Motion -- 2.3.3 Regimes of Sedimentation -- 2.3.4 Stokes' Law -- 2.3.5 Settling with Concentrated Solids -- 2.4 Process Functions -- 2.5 Summary -- References -- Problems -- Chapter 3 Batch and Semi-Batch Centrifuges -- 3.1 Spintube -- 3.2 Centrifugal Filter -- 3.3 Ultracentrifuges -- 3.3.1 Analytical Ultracentrifuge -- 3.3.2 Preparative Ultracentrifuge -- 3.3.3 Centrifugal Elutriation -- 3.4 Tubular Centrifuge -- 3.4.1 General Tubular Bowl Geometry -- 3.4.2 Ribs and Solids Scraper -- 3.4.3 Plunger Cake Discharge -- 3.4.4 Submerged Hub -- 3.5 Summary -- References -- Problems -- Chapter 4 Disk Centrifuge -- 4.1 Lamella/Inclined Plate Settler -- 4.1.1 Inclined Plate Settler Principle -- 4.1.2 Complications in Inclined Plate Settler -- 4.2 Disk-Stack Centrifuge -- 4.2.1 General Disk Geometry -- 4.2.2 Disk Angle -- 4.2.3 Disk Spacing -- 4.2.4 Process Functions of Disk Centrifuge -- 4.2.5 Feed Solids -- 4.2.6 Manual Disk Centrifuge -- 4.2.7 Intermittent Discharge -- 4.2.8 Chamber Bowl -- 4.2.9 Nozzle Discharge -- 4.2.10 Liquid Discharge -- 4.3 Feed Inlet and Accelerator -- 4.3.1 Introduction to Low Shear -- 4.3.2 Hydro-Hermetic Feed Design -- 4.3.3 Power Loss -- 4.3.4 Feed Acceleration Visual and Quantitative Testing -- 4.3.5 Improved Feed Accelerator -- 4.4 Other Considerations -- 4.4.1 Materials of Construction -- 4.4.2 Clean-in-Place -- 4.4.3 Steam Sterilization -- 4.4.4 Containment -- 4.4.5 Surface Finish -- 4.4.6 Temperature Control -- 4.4.7 Water Requirements -- 4.4.8 Noise Level -- 4.4.9 Explosion-Proof Design -- 4.5 Examples of Commercial Disk-Stack Centrifuge -- 4.6 Summary -- References -- Problems -- Chapter 5 Decanter Centrifuge -- 5.1 Solid Bowl or Decanter Centrifuge -- 5.2 Feed Rate -- 5.3 Pool Depth -- 5.4 Rotation Speed and G-force -- 5.5 Differential Speed -- 5.6 Sedimentation Enhancement |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Description based on print version record |
| Subject |
Biotechnology -- Methodology.
|
|
Centrifugation.
|
|
Centrifugation -- methods.
|
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Biotechnology -- methods.
|
| Form |
Electronic book
|
| ISBN |
0080549721 (electronic bk.) |
|
1856174778 (hbk.) |
|
9780080549729 (electronic bk.) |
|
9781856174770 (hbk.) |
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