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Book Cover
E-book
Author Hermanson, Greg T., author.

Title Bioconjugate techniques / Greg T. Hermanson
Edition Third edition
Published London : Academic Press is an imprint of Elsevier, 2013

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Description 1 online resource (xvii, 1146 pages) : illustrations (some color)
Contents Introduction to bioconjugation -- Functional targets for bioconjugation -- The reactions of bioconjugation -- Zero-length crosslinkers -- Homobifunctional crosslinkers -- Heterobifunctional crosslinkers -- Trifunctional crosslinkers -- Dendrimers and dendrons -- Cross-bridges and cleavable reagent systems -- Fluorescent probes -- (Strept)avidin-biotin systems -- Isotopic labeling techniques -- Silane coupling agents -- Microparticles and nanoparticles -- Immobilization of ligands on chromatography supports -- Buckyballs, fullerenes, and carbon nanotubes -- Chemoselective ligation; bioorthogonal reagents -- Pegylation and synthetic polymer modification -- Vaccines and immunogen conjugates -- Antibody modification and conjugation -- Liposome conjugates and derivatives -- Enzyme modification and conjugation -- Nucleic acid and oligonucleotide modification and conjugation -- Bioconjugation in the study of protein interactions
Summary Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab *Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates *Features full color illustrations *Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented
Notes Revised edition of: Bioconjugate techniques / Greg T. Hermanson. 2nd edition. 2008
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from electronic title page (ScienceDirect, viewed April 9, 2018)
Subject Bioconjugates -- Synthesis
Biochemistry -- methods
Nucleic Acids -- chemistry
Peptides -- chemistry
Proteins -- chemistry
SCIENCE -- Life Sciences -- Biochemistry.
Biochemie
Methode
Nucleinsäuren
Peptide
Proteine
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Preceded by: Hermanson, Greg T. Bioconjugate techniques. 2nd edition. 2008
ISBN 9780123822406
0123822408
9781299753839
1299753833
0123822394
9780123822390