| Description |
1 online resource (x, 290 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1431 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1431. 1064-3745
|
| Contents |
New advances in chromosome architecture / Mark C. Leake -- Single-molecule narrow-field microscopy of protein-DNA binding dynamics in glucose signal transduction of live yeast cells / Adam J.M. Wollman and Mark C. Leake -- Single-molecule imaging to characterize the transport mechanism of the nuclear pore complex / Grace Jeremy, James Stevens, and Alan R. Lowe -- Using fluorescence recovery after photobleaching (FRAP) to study dynamics of the structural maintenance of chromosome (SMC) complex in vivo / Anjana Badrinarayanan and Mark C. Leake -- Imaging DNA structure by atomic force microscopy / Alice L.B. Pyne and Bart W. Hoogenboom -- Investigating bacterial chromosome architecture using epifluorescence microscopy / Christian Lesterlin and Nelly Duabrry -- Transverse magnetic tweezers allowing coincident epifluorescence microscopy on horizontally extended DNA / Stephen J. Cross, Claire E. Brown, and Christoph G. Baumann -- Probing chromosome dynamics in Bacillus subtilis / Alan Koh and Heath Murray -- Systems biology approaches for understanding genome architecture / Sven Sewitz and Karen Lipkow -- In vivo and in situ replication labeling methods for super-resolution structured illumination microscopy of chromosome territories and chromatin domains / Ezequiel Miron [and others] -- DNA-protein interactions studied directly using single molecule fluorescence imaging of quantum dot tagged proteins moving on DNA tightropes / Luke Springall, Alessio V. Inchingolo, and Neil M. Kad -- Escherichia coli chromosome copy number measurement using flow cytometry analysis / Michelle Hawkins, John Atkinson, and Peter McGlynn -- Bacterial chromosome dynamics by locus tracking in fluorescence microscopy / Avelino Javer, Marco Cosentino Lagomarsino, and Pietro Cicuta -- Biophysical characterization of chromatin remodeling protein CHD4 / Rosa Morra [and others] -- Atomistic molecular dynamics simulations of DNA minicircle topoisomers : a practical guide to setup, performance, and analysis / Thana Sutthibutpong, Agnes Noy, and Sarah Harris -- Super-resolution microscopy and tracking of DNA-binding proteins in bacterial cells / Stephan Uphoff -- Studying the dynamics of chromatin-binding proteins in mammalian cells using single-molecule localisation microscopy / Srinjan Basu [and others] -- Intra-nuclear single-particle tracking (I-SPT) to reveal the functional architecture of chromosomes / Vincent Récamier -- Visualizing Bacillus subtilis during vegetative growth and spore formation / Xindan Wang and Paula Montero Llopis |
| Summary |
This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 16, 2016) |
| Subject |
Chromosomes -- Laboratory manuals
|
|
Chromosomes.
|
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Chromosomes
|
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Medical genetics.
|
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Medical -- Genetics.
|
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Chromosomes
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Leake, Mark C., editor.
|
| ISBN |
9781493936311 |
|
149393631X |
|
1493936298 |
|
9781493936298 |
|
