Title Cancer gene profiling : methods and protocols / edited by Robert Grützmann and Christian Pilarsky

Published New York, N.Y. : Humana Press, ©2010

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Description 1 online resource (xi, 450 pages) : illustrations Series Springer protocols Methods in molecular biology, 1940-6029 ; 576 Springer protocols (Series) Methods in molecular biology (Clifton, N.J.) ; v. 576 Contents Organizational issues in providing high-quality human tissues and clinical information for the support of biomedical research / Walter C. Bell, Katherine C. Sexton, and William E. Grizzle -- Manual microdissection / Glen Kristiansen -- Laser microdissection / Anja Rabien -- Tissue microarrays / Ana-Maria Dancau [and others] -- A decade of cancer gene profiling : from molecular portraits to molecular function / Henri Sara, Olli Kallioniemi, and Matthias Nees -- Mining expressed sequence tag (EST) libraries for cancer-associated genes / Armin O. Schmitt -- Automated fluorescent differential display for cancer gene profiling / Jonathan D. Meade [and others] -- Manual microdissection combined with antisense RNA-longSAGE for the analysis of limited cell numbers / Jutta Lüttges, Stephan A. Hahn, and Anna M. Heidenblut -- Quantitative DNA methylation profiling on a high-density oligonucleotide microarray / Anne Fassbender [and others] -- Single-nucleotide polymorphism (SNP) analysis to associate cancer risk / Julie Earl and William Greenhalf -- Application of proteomics in cancer gene profiling : two-dimensional difference in gel electrophoresis (2D-DIGE) / Deepak Hariharan, Mark E. Weeks, and Tatjana Crnogorac-Jurcevic -- Search for and identification of novel tumor-associated autoantigens / Karsten Conrad [and others] -- Integrative oncogenomic analysis of microarray data in hematologic malignancies / Jose A. Martínez-Climent [and others] -- Cancer gene profiling in pancreatic cancer / Felip Vilardell and Christine A. Iacobuzio-Donahue -- Cancer gene profiling in prostate cancer / Adam Foye and Phillip G. Febbo -- Cancer gene profiling for response prediction / B. Michael Ghadimi and Marian Grade -- The EGFR pathway as an example for genotype : phenotype correlation in tumor genes / Ulrike Mogck, Eray Goekkurt, and Jan Stoehlmacher -- Quantitation of CD39 gene expression in pancreatic tissue by real-time polymerase chain reaction / Martin Loos, Beat Künzli, nad Helmut Friess -- Functional profiling methods in cancer / Joaquín Dopazo -- Calibration of microarray gene-expression data / Hans Binder, Stephan Preibisch, and Hilmar Berger -- Meta-analysis of cancer gene-profiling data / Xinan Yang and Xiao Sun -- Target gene discovery for novel therapeutic agents in cancer treatment / Ole Ammerpohl, Sanjay Tiwari, and Holger Kalthoff Summary Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research Analysis neoplasms genen genes dna sequencing laboratoriummethoden laboratory methods protocollen protocols Cellular Biology Celbiologie Bibliography Includes bibliographical references and index Notes English Print version record Subject Cancer genes. DNA microarrays. Methodology. Biology. Diseases. Genetics -- Technique. Life sciences. Physical sciences. Laboratory Manuals Microarray Analysis Neoplasms Genetics Methods Publication Formats Biology Microchip Analytical Procedures Investigative Techniques Disease Genetic Techniques Chemistry Techniques, Analytical Biological Science Disciplines Analytical, Diagnostic and Therapeutic Techniques and Equipment Publication Characteristics Natural Science Disciplines Disciplines and Occupations Gene Expression Profiling Genes, Neoplasm methodology. biology. disease. illness. biological sciences. physical sciences. Physical sciences Methodology Life sciences Genetics -- Technique Diseases Biology Cancer genes DNA microarrays Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Grützmann, Robert Pilarsky, Christian ISBN 1597455458 9781597455459

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