| Description |
1 online resource (xi, 264 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 170 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 170
|
| Contents |
DNA Microarrays : History and Overview / Edwin M Southern -- Gel-Immobilized Microarrays of Nucleic Acids and Proteins : Production and Application for Macromolecular Research / Jordanka Zlatanova and Andrei Mirzabekov -- Sequencing by Hybridization Arrays / Radoje Drmanac and Snezana Drmanac -- Ethical Ramifications of Genetic Analysis Using DNA Arrays / Wayne W Grody -- Photolithographic Synthesis of High-Density Oligonucleotide Arrays / Glenn H McGall and Jacqueline A. Fidanza -- Automated Genotyping Using the DNA MassArrayTMT echnology / Christian Jurinke [and others] -- Ink-Jet-Deposited Microspot Arrays of DNA and Other Bioactive Molecules / Patrick Cooley [and others] -- Printing DNA Microarrays Using the Biomek 2000 Laboratory Automation Workstation / David W. Galbraith [and others] -- Hybridization Analysis of Labeled RNA by Oligonucleotide Arrays / Ulrich Certa, Antoine de Saizieu, and Jan Mous -- Analysis of Nucleic Acids by Tandem Hybridization / Rogelio Maldonado-Rodriguez and Kenneth L. Beattie -- DNA Sequencing by Hybridization with Arrays of Samples or Probes / Radoje Drmanac [and others] -- Using Oligonucleotide Scanning Arrays to Find Effective Antisense Reagents / Muhammad Sohail and Edwin M. Southern -- Low-Resolution Typing of HLA-DQA1 Using DNA Microarray / Sarah H. Haddock [and others] -- Gene Expression Analysis on Medium-Density Oligonucleotide Arrays / Ralph Sinibaldi [and others] -- Use of Bioinformatics in Arrays / Peter Kalocsai and Soheil Shams -- Confocal Scanning of Genetic Microarrays / Arthur E. Dixon and Savvas Damaskinos -- Business Aspects of Biochip Technologies / Kenneth E. Rubenstein and Dat D Dao |
| Summary |
Microarray technology, which permits the rapid, simultaneous, and highly sensitive analysis of large numbers of biological samples, is now coming into widespread use for advanced research on gene expression, mutation analysis, proteomics, and gene sequencing. In DNA Arrays: Methods and Protocols, Jang Rampal and a authoritative panel of researchers, engineers, and technologists explain in detail how to design and construct these DNA arrays, as well as how to hybridize them with biological samples for analysis. In step-by-step instructions these experts detail not only how to attach or print arrays on various matrices, but also biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies, and data collection and analysis (bioinformatics). Additional topics covered include genotyping, sequencing by hybridization, antisense reagents, HLA-DQA typing techniques, and gene expression analysis. Rounding out the technical presentation are three chapters that review the history of microarrays, the ethical ramifications of genetic analysis using DNA arrays, and the business aspects of biochip technologies. Forward-looking and state-of-the-art, DNA Arrays: Methods and Protocols provides all investigators engaged in biological and biomedical research the full range of effective, readily reproducible microarray techniques needed today to analyze on a large scale the many different genes and gene sequences now available from the Human Genome Project |
| Notes |
Source of title; title from title screen |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
|
English |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
DNA microarrays -- Laboratory manuals
|
|
Oligonucleotide Array Sequence Analysis
|
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SCIENCE -- Life Sciences -- Genetics & Genomics.
|
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DNA microarrays
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Rampal, Jang B
|
| ISBN |
9781592592340 |
|
1592592341 |
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0585418969 |
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9780585418964 |
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9786610830374 |
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6610830371 |
|
1280830379 |
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9781280830372 |
|
