| Description |
1 online resource (xi, 195 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1940-6029 ; 1169 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1169. 1064-3745
|
| Contents |
Detection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-like Receptors -- Nucleic Acid Recognition in Dendritic Cell -- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems -- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro -- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes -- Mapping of Optimal CD8 T Cell Epitopes -- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection -- MicroRNA Methodology: Advances in miRNA-technologies -- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) -- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus -- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice -- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice -- Bifunctional siRNAs for Tumor Therapy |
| Summary |
Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources |
| Analysis |
biomedische wetenschappen |
|
biomedicine |
|
moleculaire genetica |
|
molecular genetics |
|
humane genetica |
|
human genetics |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed July 8, 2014) |
| Subject |
Medical genetics -- Laboratory manuals
|
|
Natural immunity -- Laboratory manuals
|
|
Natural immunity.
|
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DNA -- immunology
|
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RNA -- immunology
|
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Immunity, Innate
|
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Toll-Like Receptors -- immunology
|
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Medical genetics
|
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Natural immunity
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Anders, Hans-Joachim, 1945- editor.
|
|
Migliorini, Adriana, editor
|
| LC no. |
2014941619 |
| ISBN |
9781493908820 |
|
1493908820 |
|
1493908812 |
|
9781493908813 |
|
