| Description |
1 online resource (xvi, 391 pages, 9 unnumbered pages of plates) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 507 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 507
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Springer protocols (Series)
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| Contents |
DNA methylation : an introduction to the biology and the disease-associated changes of a promising biomarker / Jörg Tost -- Quantification of global DNA methylation by capillary electophoresis and mass spectrometry / Maria Berdasco, Mrio F. Fraga, and Manel Esteller -- Methyl group acceptance assay for the determination of global DNA methylation levels / Kenneth P. Nephew, Curt Balch, and David G. Skalnik -- Immunodetection array / Johannes Pröll [and others] -- Methylated DNA immunoprecipitation (MeDIP) / Fabio Mohn [and others] -- The MIRA method for DNA methylation analysis / Tibor A. Rauch and Gerd P. Pfeifer -- The HELP assay / Mayumi Oda and John M. Greally -- Differential methylation hybridization : profiling DNA methylation with a high-density CpG island microarray / Pearlly S. Yan [and others] -- Analysis of DNA methylation by amplification of intermethylated sites (AIMS) / Mireia Jordà [and others] -- Methylation-sensitive representational difference analysis (MS-RDA) / Toshikazu Ushijima and Satoshi Yamashita -- Restriction landmark genomic scanning : analysis of CpG islands in genomes by 2D gel elctrophoresis / Joseph F. Costello [and others] -- GoldenGate assay for DNA methylation profiling / Marina Bibikova and Jian-Bing Fan -- 5'-azacytidine expression arrays / Paul Cairns -- DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones / Yingying Zhang [and others] -- Identification and quantification of differentially methylated loci by the Pyrosequencing technology / Emelyne Dejeux [and others] -- Mass spectrometric analysis of cytosine methylation by base-specific cleavage and primer extension methods / Dirk van den Boom and Mathias Ehrich -- Melting curve assays for DNA methylation analysis / Tomasz K. Wojdacz and Alexander Dobrovic -- Methylatioin SNaPshot : a method for the quantification of site-specific DNA methylation levels / Zachary Kaminsky and Arturas Petronis -- Bio-COBRA : absolute quantification of DNA methylation in electrofluidics chips / Romulo Martin Brena and Christoph Plass -- Restriction digestion and real-time PCR (qAMP) / Christopher C. Oakes [and others] -- MethylQuant : a real-time PCR-based method to quantify DNA methylation at single specific cytosines / Claire Dugast-Darzacq and Thierry Grange -- Methylation-specific PCR / Julien D.F. Licchesi and James G. Herman -- MethyLight / Mihaela Campan [and others] -- Quantification of methylated DNA by HeavyMethyl duplex PCR / Jürgen Distler -- Analysis of methylated circulating DNA in cancer patients' blood / Eiji Sunami [and others] -- Prevention of PCR cross-contamination by UNG treatment of bisulfite-treated DNA / Reimo Tetzner -- Profiling DNA methylation from small amounts of genomic DNA starting material : efficient sodium bisulfite conversion and subsequent whole-genome amplification / Jonathan Mill and Arturas Petronis |
| Summary |
Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to this fact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, DNA Methylation: Methods and Protocols, Second Edition is the perfect resource for scientists who wish to further our understanding of the molecular processes that determine the genomic DNA methylation landscape and for those who aim to identify and implement DNA methylation-based biomarkers for clinical and diagnostic applications |
| Analysis |
dna methylation |
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moleculaire biologie |
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molecular biology |
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Nucleic Acids |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
DNA -- Methylation
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DNA Methylation
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DNA -- Methylation
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Tost, Jörg.
|
| ISBN |
9781597455220 |
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1597455229 |
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