Title Forensic DNA profiling protocols / edited by Patrick J. Lincoln and Jim Thomson

Published Totowa, N.J. : Humana Press, ©1998

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Description 1 online resource (x, 309 pages) : illustrations Series Methods in molecular biology ; v. 98 Methods in molecular biology (Clifton, N.J.) ; v. 98 Contents Recovery of high-molecular-weight DNA from blood and forensic specimens / Peter M. Schneider -- Recovery of DNA for PCR amplification from blood and forensic samples using a chelating resin / Jeanne M. Willard, Dennis A. Lee, Mitchell M. Holland -- PCR analysis of DNA from fresh and decomposed bodies and skeletal remains in medicolegal death investigations / Manfred N. Hochmeister -- PCR analysis from cigaret butts, postage stamps, envelope sealing flaps, and other saliva-stained material / Manfred N. Hochmeister, Oskar Rudin, Edda Ambach -- Quantification of DNA by slot-blot analysis / Julia Andersen -- Rapid assessment of PCR product quality and quantity by capillary electrophoresis / John M. Butler -- Southern blotting of genomic DNA for DNA profiling / Jim Thomson -- Preparation and use of alkaline-phosphatase-conjugated oligonucleotide probes for single-locus and multilocus VNTR analysis / Wayne P. Childs, Michael B. Webb, Gillian Rysiecki -- Preparation and use of 32P-labeled single-locus VNTR probes in identity testing / Jürgen Henke, Lotte Henke -- Statistical methods employed in evaluation of single-locus probe results in criminal identity cases / Bruce S. Weir -- Interpretation and statistical evaluation of multilocus DNA fingerprints in paternity and relationship testing / Gillian Rysiecki, John F. Brookfield -- Overview of PCR-based systems in identity testing / Bernd Brinkmann -- An introduction to PCR primer design and optimization of amplification reactions / James M. Robertson, Jennifer Walsh-Weller -- Analysis of amplified fragment-length polymorphisms (VNTR/STR loci) for human identity testing / Bruce Budowle, Robert C. Allen -- Amplification of short tandem repeat loci using PCR / Niels Morling -- Manual electrophoretic methods for genotyping amplified STR loci / Chris P. Phillips, Angel Carracedo, Maviky V. Lareu -- Genotyping STR loci using an automated DNA sequencer / David Watts -- Separation of PCR fragments by means of direct blotting electrophoresis / Lotte Henke, Jürgen Henke -- Amplification and sequencing of mitochondrial DNA in forensic casework / Robert J. Steighner, Mitchell Holland -- Minisatellite variant repeat unit mapping using PCR (MVR-PCR) / David L. Neil -- Sex determination by PCR analysis of the X-Y amelogenin gene / Atsushi Akane -- Species determination by analysis of the cytochrome b gene / Rita Barallon -- Use of the AmpliType PM + HLA DQA1 PCR amplification and typing kits for identity testing / Michael L. Baird -- The use of capillary electrophoresis in genotyping STR loci / John M. Butler -- Solid-phase minisequencing as a tool to detect DNA polymorphism / Ann-Christine Syvänen Summary In Forensic DNA Profiling Protocols, leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR-based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct-phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing. Forensic DNA Profiling Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science. The readily reproducible methods elucidated here represent the state-of-the-art for those scientists who want to introduce, establish, and perfect DNA identification techniques in their laboratories Analysis forensische wetenschap forensic science dna identificatie identification genetische merkers genetic markers polymerase chain reaction biologische technieken biological techniques genotyping moleculaire merkers molecular markers biomaterialen biomaterials protocollen protocols Nucleic Acids Bibliography Includes bibliographical references and index Notes Print version record In Springer Protocols Subject Forensic genetics -- Laboratory manuals DNA fingerprinting -- Laboratory manuals Polymerase chain reaction -- Laboratory manuals DNA -- Analysis -- Laboratory manuals Medical jurisprudence. DNA Fingerprinting -- methods DNA -- chemistry Forensic Medicine forensic medicine. MEDICAL -- Forensic Medicine. Medical jurisprudence DNA -- Analysis DNA fingerprinting Forensic genetics Polymerase chain reaction Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Lincoln, Patrick J. Thomson, Jim, 1963- ISBN 9781592592043 159259204X 0585239797 9780585239798 0896034437 9780896034433

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