Title DNA topoisomerase protocols. Volume 94, Part 1, DNA topology and enzymes / edited by Mary-Ann Bjornsti and Neil Osheroff

Published Totowa, N.J. : Humana Press, ©1999

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Description 1 online resource (xv, 327 pages) : illustrations Series Methods in molecular biology ; v. 94, pt. 1 Methods in molecular biology (Clifton, N.J.) ; volume 94, pt. 1. Contents Introduction to DNA topoisomerases / Mary-Ann Bjornsti, Neil Osheroff -- Resolution of DNA molecules by one-dimensional agarose-gel electrophoresis / Mary-Ann Bjornsti, Maureen D. Megonigal -- Two-dimensional agarose-gel electrophoresis of DNA topoisomers / Ryo Hanai, Joaquim Roca -- Analysis of altered DNA structures: cruciform DNA / Albert J. Courey -- Purification of supercoiled plasmid DNA / Kelly L. Jordan, Devon L. Evans, David J. Hall -- Purification and use of DNA minicircles with different linking numbers / Giorgio Camilloni, Rodolfo Negri, Micaela Caserta, Ernesto Mauro -- Isolation of kinetoplast DNA / Theresa A. Shapiro, Viiu A. Klein, Paul T. Englund -- Isolation of knotted DNA from coliphage P4 / Morten Isaksen, Bryan Julien, Richard Calendar, Björn H. Lindqvist -- Analysis of DNA knots and catenanes by agarose-gel electrophoresis / Stephen D. Levene, Hua Tsen -- Sedimentation analysis of bacterial nucleoid structure / Karl Drlica, Chang-Rung Chen, Samuel Kayman -- Coating DNA with RecA protein to distinguish DNA path by electron microscopy / E. Lynn Zechiedrich, Nancy J. Crisona -- Methods for analyzing DNA bending / Jason D. Kahn -- Formation of extrachromosomal DNA rings in Saccharomyces cerevisiae using site-specific recombination / Marc R. Gartenberg -- Overexpression and purification of bacterial DNA gyrase / Anthony Maxwell, Alison J. Howells -- Overexpression and purification of bacterial DNA topoisomerase I / Chang-Xi Zhu, Yuk-Ching Tse-Dinh -- Overexpression and purification of Escherichia coli DNA topoisomerase III / Russell J. DiGate -- Overexpression and purification of bacterial topoisomerase IV / Hong Peng, Kenneth J. Marians -- Purification of the bacteriophage T4 type II DNA topoisomerase / Kenneth N. Kreuzer, Sue H. Neece -- Overexpression and purification of DNA topoisomerase I from yeast / Mary-Ann Bjornsti, Jolanta Fertala -- Overexpression and purification of Saccharomyces cerevisiae DNA topoisomerase II from yeast / Janet E. Lindsley -- Purification of DNA topoisomerase II from Drosophila melanogaster / Stacie J. Froelich-Ammon, Paul S. Kingma, Neil Osheroff -- Purification of DNA topoisomerase I from human placenta / Joseph A. Holden -- Purification of baculovirus-expressed human DNA topoisomerase I / Lance Stewart, James J. Champoux -- Rapid purification of DNA topoisomerase II containing a hexahistidine tag by metal ion affinity chromatography / Harald Biersack, Sanne Jensen, Ole Westergaard -- Metabolic labeling, immunoprecipitation, and two-dimensional tryptic phosphopeptide mapping of human topoisomerase II / Nicholas J. Wells, Ian D. Hickson -- Immunoblot analysis and band depletion assays / Scott H. Kaufmann, Phyllis A. Svingen -- Visualization of DNA topoisomerases by electron microscopy / Piero Benedetti, Alessandra Silvestri -- Compendium of DNA topoisomerase sequences / Paul R. Caron Summary DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and antibacterial agents, and about DNA repair Analysis dna isomerasen isomerases genexpressie gene expression biologische technieken biological techniques topologie topology protocollen protocols Nucleic Acids Bibliography Includes bibliographical references and index Notes Print version record Subject DNA -- Structure -- Laboratory manuals DNA -- Conformation -- Laboratory manuals DNA topoisomerases. DNA topoisomerase I. DNA Topoisomerases, Type I DNA -- chemistry DNA -- drug effects DNA -- physiology DNA Topoisomerases SCIENCE -- Life Sciences -- Genetics & Genomics. DNA topoisomerase I DNA -- Conformation DNA -- Structure DNA topoisomerases Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Osheroff, Neil Bjornsti, Mary-Ann ISBN 9781592592593 1592592597 0585243999 9780585243993 9780896034440 0896034445

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