High-throughput RNAi screening : methods and protocols / editors David O. Azorsa, Institute of Molecular Medicine, Phoenix Children's Hospital, Phoenix, Arizona, USA ; Shilpi Arora, Constellation Pharmaceuticals, Cambridge, Massachusetts, USA
Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening -- Optimization of Transfection Conditions for siRNA Screening -- High Throughput siRNA Screening Using Reverse Transfection -- Genome-Wide siRNA Screening Using Forward Transfection: Identification of Modulators of Membrane Trafficking in Mammalian Cells -- Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform -- A Protocol for a High-Throughput Multiplex Cell Viability Assay -- RNAi Screening of Leukemia Cells Using Electroporation -- Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening -- In Vitro Pooled shRNA Screening to Identify Determinants of Radiosensitivity -- Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens -- In Vitro High Throughput RNAi Screening to Accelerate the Process of Target Identification and Drug Development -- High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans -- Design and Methods of Large-Scale RNA Interference Screens in Drosophila -- Genome-Wide RNAi Screening in C. elegans for Aging and Innate Immunity -- RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae -- RNAi Screening in Spodoptera frugiperda -- Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos -- Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens -- Data Analysis For High Throughput RNAi Screening
Summary
High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries
Bibliography
Includes bibliographical references and index
Notes
Online resource; title from PDF title page (SpringerLink, viewed September 22, 2016)
