| Description |
1 online resource (xi, 278 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; volume no. 1507 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1507. x 1064-3745
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| Contents |
Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators / Ashish Misra and Michael R. Green -- Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins / Sophie Beyer, Philippe Robin, and Slimane Ait-Si-Ali -- Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos / George E. Gentsch and James C. Smith -- Systematic Discovery of Chromatin-Bound Protein Complexes from ChIP-seq Datasets / Eugenia Giannopoulou and Olivier Elemento -- Assay for Transposase-Accessible Chromatin with High-throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos / Canan Doganli, Melissa Sandoval, Sean Thomas, and Daniel Hart -- Establishment of Time- and Cell-Specific RNAi in Caenorhabditis elegans / Masayuki Hamakawa and Takaaki Hirotsu -- Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing / Bharathi Suresh, Suresh Ramakrishna, and Hyongbum Kim -- Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets / Jia Zhang and Klaus H. Kaestner -- eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response / Ji-Ye Yin, Zizheng Dong, and Jian-Ting Zhang -- High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine / Andrzej J. Rutkowski and Lars Dölken -- Accurate Detection of Differential Expression and Splicing Using Low-Level Features / Tomi Suomi and Laura L. Elo -- Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry / Mowei Zhou, Si Wu, David L. Stenoien, Zhaorui Zhang, Lanelle Connolly, Michael Freitag, and Ljiljana Paša-Tolić -- Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae / Marcus J.O. Johansson -- Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency : Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract / Mihwa Na, Susana T. Valente, and Kevin Ryan -- Unbiased Interrogation of 3D Genome Topology Using Chromosome Conformation Capture Coupled to High-Throughput Sequencing (4C-Seq) / Rutger W.W. Brouwer, Mirjam C.G.N. van den Hout, Wilfred F.J. van IJcken, Eric Soler, and Ralph Stadhouders -- Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells / Krishna Mohan Parsi, Erica Hennessy, Nicola Kearns, and René Maehr -- In Vitro Assay to Study Histone Ubiquitination During Transcriptional Regulation / Jogender Tushir-Singh and Sanchita Bhatnagar -- Determination of Alternate Splicing Events Using Transcriptome Arrays / Rebecca S. DeVaux, Marcy Kuentzel, Jason Herschkowitz, and Sridar V. Chittur -- Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene / Matteo Forloni, Thuy Ho, Lisha Sun, and Narendra Wajapeyee -- Transcriptional Analysis-based Integrative Genomics Approach to Identify Tumor Promoting Metabolic Genes / Romi Gupta and Narendra Wajapeyee |
| Summary |
This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed November 21, 2016) |
| Subject |
Genetic regulation -- Laboratory manuals
|
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Eukaryotic cells -- Genetics -- Laboratory manuals
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Genetic regulation.
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Genetic transcription.
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Eukaryotic cells.
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Gene Expression Regulation
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Transcription, Genetic
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Eukaryotic Cells
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Medical genetics.
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Medical -- Genetics.
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Genetic transcription
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Eukaryotic cells
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Eukaryotic cells -- Genetics
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Genetic regulation
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Wajapeyee, Narendra, editor.
|
|
Gupta, Romi, editor
|
| ISBN |
9781493965182 |
|
1493965182 |
|
1493965166 |
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9781493965168 |
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