| Description |
1 online resource (xiv, 441 pages) : illustrations |
| Series |
Methods in molecular medicine ; 30 |
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Methods in molecular medicine ; 30.
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| Contents |
pt. I. Genetics in Vascular Disease. 1. Detection of Mutations and DNA Polymorphisms in Genes Involved in Cardiovascular Diseases by Polymerase Chain Reaction -- Single-Strand Conformation Polymorphism Analysis / Shu Ye and Adriano M. Henney. 2. Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis / Ana Cenarro, Fernando Civeira and Miguel Pocovi. 3. Radiation Hybrid (RH) Mapping of Human Smooth Muscle-Restricted Genes / Joseph M. Miano, Emilio Garcia and Ralf Krahe -- pt. II. Isolation of Genes Expressed in Vascular Tissue. 4. Efficient Extraction of RNA from Vascular Tissue / Catherine F. Townsend, Christopher M.H. Newman and Sheila E. Francis. 5. Preparation of cDNA Libraries from Vascular Cells / Mark E. Lieb and Mark B. Taubman. 6. Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells / Csilla Csortos, Virginie Lazar and Joe G.N. Garcia. 7. Cloning Full-Length cDNAs from Vascular Tissues and Cells by Rapid Amplification of cDNA Ends (RACE) and RT-PCR / Rong-Fong Shen. 8. Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques / Kerry Tyson and Catherine Shanahan. 9. The Use of Differential mRNA Display (DDRT-PCR) to Identify Genes Differentially Expressed in Normal and Diseased Vascular Cells / Paul J. Adam. 10. Identification of Novel Protein Kinases in Vascular Cells / Ian Zachary, Spiros Servos and Barbara Herren -- pt. III. Methods for Mapping Transcriptional Start Sites and Measurement of Promoter Activity in Vascular Cells. 11. Primer Extension Analysis to Map Transcription Start Sites of Vascular Genes / Yutaka Kitami and Kunio Hiwada. 12. Use of Liposome-Mediated DNA Transfection to Determine Promoter Activity in Smooth Muscle Cells / Rosalind P. Fabunmi. 13. Nuclear Run-On Assay to Study Gene Transcription in Vascular Cells / Ulrich Laufs and James K. Liao. 14. Electromobility Shift Analysis (EMSA) Applied to the Study of NF-kappa B Binding Interactions in Vascular Smooth Muscle Cells / Todd Bourcier -- pt. IV. Molecular Analysis of mRNA Expression in Vascular Cells. 15. Measurement of Gene Expression in the Vascular Wall by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis / Joachim Fruebis. 16. Northern Blot Analysis to Quantify Gene Expression in Vascular Diseases / Mingyi Chen and Tatsuya Sawamura. 17. Localization of Gene Expression in Human Atherosclerotic Lesions by In Situ Hybridization / Lee D.K. Buttery and Julia M. Polak. 18. RNase Protection Assays for Quantitation of Gene Expression in Vascular Tissue / Nengyu Yang, Shuli Wang and James E. Faber -- pt. V. Molecular Methods to Study Apoptosis and Phenotypic Changes in Vascular Cells and Tissue. 19. Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL) / Mark Kockx, Johannes Muhring and Michiel Knaapen. 20. In Vitro Detection of Apoptosis in Isolated Vascular Cells / Shiu-Wan Chan and Martin R. Bennett. 21. Molecular Assessment of the Phenotypic Changes Associated with Smooth Muscle Cells Using Two-Dimension Electrophoresis and Microsequence Analysis / Rachel Johnatty, Giulio Gabbiani and Pascal Neuville -- pt. VI. In Vitro Methods to Express Foreign Genes in Vascular Cells. 22. Simple Methods for Preparing Recombinant Adenoviruses for High-Efficiency Transduction of Vascular Cells / Stuart A. Nicklin and Andrew H. Baker. 23. Generation of Recombinant Adeno-Associated Viruses for Delivery of Genes into Vascular Cells / Carmel M. Lynch. 24. Hemagglutinating Virus of Japan Liposome-Mediated Gene Delivery to Vascular Cells / Yoshikazu Yonemitsu and Yasufumi Kaneda. 25. High-Efficiency and Low-Toxicity Adenovirus-Assisted Endothelial Transfection / Takamune Takahashi, Keiko Takahashi and Thomas O. Daniel. 26. Gene Transfer in Vascular Cells Using an Engineered Na-H Exchanger (NHE1) as a Selectable Market / Jacques Pouyssegur and Daniele Roux. 27. Use of Retroviruses to Express Exogenous Genes in Vascular Smooth Muscle Cells / Trevor D. Littlewood and Stella A. Pelengaris. 28. Embryonal Stem (ES) Cell-Derived Macrophages: A Cellular System that Facilitates the Genetic Dissection of Macrophage Function / Kathryn J. Moore and Mason W. Freeman. 29. Delivery of Recombinant Adenoviruses to Human Saphenous Vein / Sarah J. George and Andrew H. Baker -- pt. VII. In Vivo Vascular Gene Transfer Protocols. 30. An In Vivo Angiogenesis Assay to Study Positive and Negative Regulators of Neovascularization / Maurizio C. Capogrossi and Antonino Passaniti. 31. Efficient Liposome-Mediated Gene Transfer to Rabbit Carotid Arteries in Vivo / Michael-Christopher Keogh and Daxin Chen. 32. Gene Delivery to Rabbit Arteries Using the Collar Model / Mikko O. Hiltunen, Mikko P. Turunen and Seppo Yla-Herttuala. 33. Delivery of Antisense Oligonucleotides to the Vascular Wall / Cathy M. Holt, Julian Gunn and Darren Lambert / [and others]. 34. Local Gene Delivery of Recombinant Adenoviruses to the Rat Carotid Artery In Vivo / Clare M. Dollery and Jean R. McEwan |
| Summary |
In Vascular Disease: Molecular Biology and Gene Therapy Protocols, Andrew Baker and a noted panel of expert investigators describe today's most powerful molecular methods for investigating the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that have been used successfully to identify specific mutations involved in cardiovascular disorders, to those for transferring genes associated with cardiovascular disease into various vascular cell types by in vitro and in vivo routes. There are methods to identify novel genes and generate full-length cDNAs, to study gene transcription and promoter activity easily and effectively, and to ascertain precisely gene expression levels within the individual cell types in different pathophysiological conditions. Accurate methods to quantify apoptosis in both cultured cells and pathological specimens are also given. Vascular Disease: Molecular Biology and Gene Therapy Protocols offers today's vascular biologist and gene therapist an unprecedented ability to study the pathogenesis of vascular disease and readily to probe the potential for gene-based therapies. Powerful and productive, the techniques presented here operate across a wide range of exciting research areas, and promise spectacular therapeutic breakthroughs in the ongoing battle against vascular disease |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Blood-vessels -- Diseases -- Molecular aspects -- Laboratory manuals
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Blood-vessels -- Diseases -- Gene therapy -- Laboratory manuals
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Blood-vessels -- Diseases -- Genetic aspects -- Laboratory manuals
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Vascular Diseases -- genetics
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Molecular Biology -- methods
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MEDICAL -- Cardiology.
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Blood-vessels -- Surgery
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Medicine
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| Genre/Form |
Electronic books
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Baker, Andrew H.
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| ISBN |
9781592592470 |
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0585246211 |
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9780585246215 |
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1592592473 |
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