| Description |
1 online resource (xiv, 577 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 126 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 126.
|
| Contents |
Drosophila as a genetic tool to define vertebrate pathway players / N.M. Bonini -- Bioassays for studying the role of the peptide growth factor activin in early amphibian embryogenesis / M. Asashima [and others] -- Analysis of mammary gland morphogenesis / C.D. Roskelley, C. Wu and A.M. Somasiri -- Specification of cardiac mesenchyme and heart morphogenesis in vitro / H.J. Yost -- Craniofacial development and patterning / H. Slavkin, G. Nuckolls and L. Shum -- Craniofacial skeletal morphogenesis in vitro / R.C. Ogle -- Skeletal morphogenesis / S. Mundlos -- Transplantation and culture techniques for the analysis of urodele limb regeneration / D.L. Stocum -- Retroviral infection of T-cell precursors in thymic organ culture / L.M. Spain, L.L. Lau and Y. Takahama -- Assay for the isolation of hepatogenic factors. Key molecules in hepatocyte formation and liver morphogenesis / J.A. Spijkers, T.B. Hakvoort and W.H. Lamers -- Skin morphogenesis. Embryonic chicken skin explant cultures / C.M. Chuong -- Apoptosis in development / L. Lin and Z.F. Zakeri -- Methods to detect patterns of cell death in drosophila / N.M. Bonini -- Mechanisms of neurulation / G.C. Schoenwolf and J.L. Smith -- Neurulation and neural tube closure defects / A. Copp [and others] -- Neural tube defects / T. Inagaki [and others] -- Experimental manipulation and morphometric analysis of neural tube development / M.E. Desmond and P.A. Haas -- Isolation of neuroepithelium and formation of minispheres / M.E. Desmond and M.C. Field -- Examination of normal and abnormal placentation in the mouse / M.R. Blackburn -- Palatal dysmorphogenesis. Palate organ culture / B.D. Abbott -- Palatal dysmorphogenesis. Quantitative RT-PCR / G.A. Held and B.D. Abbott -- Transspecies grafting as a tool to understand the basis of murine developmental limb abnormalities / S.M. Bell, C.M. Schreiner and W.J. Scott -- Assessment of laterality defects in rodent embryos / M. Fujinaga -- Cardiac morphogenesis and dysmorphogenesis. I. Normal development / A. Wessels and R. Markwald -- Analysis of two aspects of left-right patterning of the vertebrate heart. Heart tube position and heart tube chirality / A.J. Chin -- Biologically based risk assessment models for developmental toxicity / C. Lau and R.W. Setzer -- Positional cloning / L. Xu and Y. Li -- Gene trapping in embryonic stem cells in vitro to identify novel developmentally regulated genes in the mouse / G.E. Lyons [and others] -- PCR-based cloning of cortically localized RNAs from xenopus oocytes / J. Zhang and M.L. King -- Analysis of mRNA expression during preimplantation development / K.E. Latham, E. De la Casa and R.M. Schultz -- Differential screens with subtracted PCR-generated cDNA libraries from subregions of single mouse embryos / A. Shimono and R.R. Behringer -- HPLC-based mRNA differential display / T.B. Knudsen -- Production of transgenic drosophila / M. Fujioka [and others] -- Transgenic manipulation of the sea urchin embryo / J.P. Rast -- Transgenic zebrafish / S. Lin -- Production of avian chimeras and germline transmission / K.L. Woods [and others] -- Incorporation of genetically modified cells in chicken chimeras / L.D. Zajchowski [and others] -- Long-term culture of chicken blastodermal cells (cbcs) and selection of transfected cbcs using antibiotic resistance / Q. Wei, K.L. Woods and R.J. Etches -- Nuclear transplantation and cloning in mammals / K.E. Latham and M.E. Westhusin -- Production of transgenic mice / J. Richa -- Production of transgenic mice with yeast artificial chromosomes / A. Jakobovits, B.T. Lamb and K.R. Peterson -- Gene-targeting strategies / S.S. Cheah and R.R. Behringer -- Chimeric animals and germline transmission / J.S. Khillan -- Conditional gene knockout using cre recombinase / Y. Le and B. Sauer -- Application of cre/loxp in drosophila. Site-specific recombination and transgene coplacement / M.L. Siegal and D.L. Hartl |
| Summary |
In Adrenegic Receptor Protocols, Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator, and second messenger methodologies, as well as immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. Each of the experts writing here details the use of their chosen method in examining the adrenergic receptor system, using aspects of the genetic flow of information as a guide: DNA ' RNA ' transactivator ' protein expression ' second messenger analyses ' cellular analyses ' transgenic whole animal approaches. Comprehensive and rich in practical detail, Adrenergic Receptor Protocols provides the first collection of reproducible methods for the study of these important regulators of CNS-mediated behavior and neural function. Its state-of-the-art methods constitute today's gold standard reference for all neurobiologists, neurochemists, neurologists, and pharmacologists studying this exceptionally important class of receptors |
| Notes |
Source of title; title from Title page |
| Bibliography |
Includes bibliographical references and index |
| Issuing Body |
Digitization of Biomedprotocols made available on the world wide web by Humana Press |
| Notes |
English |
| Subject |
Adrenaline -- Receptors -- Laboratory manuals
|
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Genetic regulation.
|
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Receptors, Adrenergic -- analysis
|
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Gene Expression Regulation
|
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Receptors, Adrenergic -- genetics
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Sequence Analysis, RNA -- methods
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MEDICAL -- Neuroscience.
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PSYCHOLOGY -- Neuropsychology.
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Adrenaline -- Receptors
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Wetenschappelijke technieken.
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Machida, Curtis A
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Humana Press.
|
| LC no. |
99034211 |
| ISBN |
1592596843 |
|
9781592596843 |
|
0896036022 |
|
9780896036024 |
|
1280831596 |
|
9781280831591 |
|
9786610831593 |
|
6610831599 |
|
