| Description |
1 online resource (xi, 291 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; 1775 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1775. 1064-3745
|
| Contents |
Introduction : overview of fungal genomics / Ronald P. de Vries, Igor V. Grigoriev, and Adrian Tsang -- Fungal genomic DNA extraction methods for rapid genotyping and genome sequencing / Annie Bellemare, Tricia John, and Sandrine Marqueteau -- Purification of fungal high molecular weight genomic DNA from environmental samples / Laure Fauchery, Stéphane Uroz, Marc Buée, and Annegret Kohler -- Genome sequencing / Yuko Yoshinaga, Christopher Daum, Guifen He, and Ronan O'Malley -- Methods for genomic characterization and maintenance anaerobic fungi / Xuefeng Peng, Candice L. Swift, Michael K. Theodorou, and Michelle A. O'Malley -- Efficient extraction method for high quality fungal RNA from complex lignocellulosic substrates / Miia R. Mäkelä and Kristiina Hildén -- Isolation of high quality RNA for metatranscriptomic analysis of lignocellulose digestion in the rumen / R.J. Gruninger, R.J. Forster, and T.A. McAllister -- Fungal transcriptomics / Vasanth R. Singan, Rita C. Kuo, and Cindy Chen -- Mass spectrometry-based proteomics / Marcos Rafael Di Falco -- Mass spectrometry-based metabolomics / Young-Mo Kim and Heino M. Heyman -- Genome editing : CRISPR-Cas9 / Jakob B. Hoof, Christina S. Nødvig, and Uffe H. Mortensen -- Evolutionary adaptation togenerate mutants / Ronald P. de Vries, Ronnie Lubbers, Aleksandrina Patyshakuliyeva, Ad Wiebenga, and Isabelle Benoit-Gelber -- Genome assembly / Alicia Clum -- Fungal epigenomics : detection and analysis / Stephen J. Mondo, Rita C. Kuo, and Vasanth R. Singan -- Fungal genome annotation / Sajeet Haridas, Asaf Salamov, and Igor V. Grigoriev -- Manual gene curation and functional annotation / Erin McDonnell, Kimchi Strasser, and Adrian Tsang -- Evaluating programs for predicting genes and transcripts with RNA-Seq support in fungal genomes / Ian Reid -- Genomic sequence variation analysis by resequencing / Joel Martin, Wendy Schackwitz, and Anna Lipzen -- ChIP-Seq analysis in Neurospora crassa / Aileen R. Ferraro and Zachary A. Lewis -- Fungal phylogenomics / Robert Riley and Laszlo Nagy -- Phylogenetic analysis of protein family / Letian Song, Sherry Wu, and Adrian Tsang -- Parasexual crossings for bulk segregant analysis in Aspergillus niger to facilitate mutant identification via whole genome sequencing / Mark Arentshorst and Arthur F.J. Ram |
| Summary |
Annotation This volume details protocols covering nearly all aspects of fungal genomics. New and updated chapters guide the reader through experimental genomics, biotechnologies, and the analysis and processing of data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fungal Genomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol viewed June 7, 2018) |
| Subject |
Fungi -- Genetics -- Laboratory manuals
|
|
Mycology -- Laboratory manuals
|
|
Fungi -- genetics
|
|
Genome, Fungal
|
|
Microbiology (non-medical)
|
|
Science -- Life Sciences -- Biology -- Microbiology.
|
|
Fungi -- Genetics
|
|
Mycology
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Vries, Ronald P. de, editor
|
|
Tsang, Adrian, editor
|
|
Grigoriev, Igor V., editor
|
| ISBN |
9781493978045 |
|
1493978047 |
|
1493978039 |
|
9781493978038 |
|
