Title Stress response : methods and protocols / edited by Stephen M. Keyse

Published Totowa, NJ : Humana Press, ©2000

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Description 1 online resource (xvi, 488 pages) : illustrations Series Methods in molecular biology ; v. 99 Methods in molecular biology (Clifton, N.J.) ; v. 99 Contents Identifying and counting protein modifications triggered by nitrosative stress / Prabodh K. Sehajpal, Harry M. Lander -- Determination of carbonyl groups in oxidized proteins / Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, Emily Shacter -- Quantitation of 4-hydroxynonenal protein adducts / Koji Uchida, Earl R. Stadtman -- Detection of oxidative stress in lymphocytes using dichlorodihydrofluorescein diacetate / Cecile M. Krejsa, Gary L. Schieven -- The measurement of protein degradation in response to oxidative stress / Thomas Reinheckel, Tilman Grune, Kelvin J. Davies -- Analysis of the role of the AMP-activated protein kinase in the response to cellular stress / D. Grahame Hardie, Ian P. Salt, Stephen P. Davies -- Detection and activation of stress-responsive tyrosine kinases / Gary L. Schieven -- Detection of DNA-dependent protein kinase in extracts from human and rodent cells / Yamini Achari, Susan P. Lees-Miller -- Expression and assay of recombinant ATM / Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, Yosef Shiloh -- Detection and purification of a multiprotein kinase complex from mammalian cells : IKK Signalsome / Frank Mercurio, David B. Young, Anthony M. Manning -- Methods to assay stress-activated protein kinases / Ana Cuenda -- Monitoring the activation of stress-activated protein kinases using GAL4 fusion transactivators / Chao-Feng Zheng, Li Xu -- Use of kinase inhibitors to dissect signaling pathways / Ana Cuenda, Dario R. Alessi -- The development and use of phospho-specific antibodies to study protein phosphorylation / Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, Ted R. Hupp -- Peptide assay of protein kinases and use of variant peptides to determine recognition motifs / D. Grahame Hardie -- Assaying NF-KB and AP-1 DNA-binding and transcriptional activity / Judith M. Mueller, Heike L. Pahl -- Analysis of the mammalian heat-shock response : inducible gene expression and heat-shock factor activity / Anu Mathew, Yanhong Shi, Caroline Jolly, Richard I. Morimoto -- Approaches to define the involvement of reactive oxygen species and iron in ultraviolet-A inducible gene expression / Charareh Pourzand, Olivier Reelfs, Rex M. Tyrrell -- The human immunodeficiency virus LTR-promoter region as a reporter of stress-induced gene expression / Michael W. Bate, Sushma R. Jassal, David W. Brighty -- SAGE : the serial analysis of gene expression / Jill Powell -- Analysis of differential gene expression using the SABRE enrichment protocol / Daniel J. Lavery, Philippe Fonjallaz, Fabienne Fleury-Olela, Ueli Schibler -- UVB-regulated gene expression in human keratinocytes: analysis by differential display / Harry Frank Abts, Thomas Welss, Kai Breuhahn, Thomas Ruzicka -- Heme oxygenase activity current methods and applications / Stefan W. Ryter, Egil Kvam, Rex M. Tyrrell -- Analysis of molecular chaperone activities using in vitro and in vivo approaches / Brian C. Freeman, Annamieke Michels, Jaewhan Song, Harm H. Kampinga, Richard I. Morimoto -- Analysis of chaperone properties of small Hsp's / Monika Ehrnsperger, Matthias Gaestel, Johannes Buchner -- Analysis of small Hsp phosphorylation / Rainer Benndorf, Katrin Engel, Matthias Gaestel -- Analysis of multisite phosphorylation of the p53 tumor-suppressor protein bytryptic phosphopeptide mappin / David W. Meek, Diane M. Milne -- Development of physiological models to study stress protein responses / Ted R. Hupp Summary In is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research. Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Stress (Physiology) -- Laboratory manuals Molecular biology -- Laboratory manuals Cellular signal transduction -- Laboratory manuals Heat shock proteins -- Laboratory manuals Gene expression -- Laboratory manuals Cellular signal transduction. Heat shock proteins. Gene expression. Molecular Biology Signal Transduction Heat-Shock Proteins Gene Expression Stress, Physiological -- physiology SCIENCE -- Life Sciences -- Biochemistry. Cellular signal transduction Gene expression Heat shock proteins Molecular biology Stress (Physiology) Genre/Form Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Keyse, Stephen M. ISBN 9781592590544 0585395101 9780585395104 1592590543 1280820462 9781280820465 9786610820467 6610820465

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