Title Calpain methods and protocols / edited by John S. Elce

Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (xviii, 343 pages) : illustrations Series Methods in molecular biology ; v. 144 Methods in molecular biology (Clifton, N.J.) ; v. 144 Contents pt. I. Purification of Components of the Calpain System. 1. Purification of [mu]-Calpain, m-Calpain, and Calpastatin from Animal Tissues / Valery F. Thompson and Darrel E. Goll. 2. A Simple Protocol for Separation and Assay of [mu]-Calpain, m-Calpain, and Calpastatin from Small Tissue Samples / Jan-Olof Karlsson. 3. Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms From Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography / Elsa J.B. Murray, Keyvan Behnam and Mario S. Grisanti / [and others]. 4. Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose / Dorothy E. Croall. 5. Affinity Purification of [mu]-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump: Some Studies on Erythrocyte [mu]-Calpain / Maurizio Molinari and Ernesto Carafoli. 6. Expression of m-Calpain in Escherichia coli / John S. Elce Summary In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of æ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Calpain -- Laboratory manuals Cysteine proteinases. Hydrolases. Enzymes. Calpain. Cysteine Endopeptidases Cysteine Proteases Endopeptidases Peptide Hydrolases Hydrolases Enzymes Enzymes and Coenzymes Chemicals and Drugs Calpain enzyme. SCIENCE -- Life Sciences -- Biochemistry. Hydrolases Enzymes Cysteine proteinases Calpain Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Elce, John S. ISBN 9781592590506 0896036324 9780896036321 1592590500 128082042X 9781280820427 9786610820429 6610820422

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