| Description |
1 online resource |
| Contents |
Title Page -- Copyright Page -- Contents -- Preface -- Acknowledgments -- About the Companion Website -- Introduction -- Module 1 Working with and Learning About Common Laboratory Techniques and Equipment -- Exercise 1A: Using Common Laboratory Tools to Evaluate Measurements Pre-laboratory Thinking Questions -- Directions -- Exercise 1B: Using Common Laboratory Tools to Evaluate Measurements -- Measurements Introduction -- Let's Put This to Practice! -- Working with Serological Pipettes and Micropipettes -- Let's Put This to Practice! -- Practice Pipetting Small Volumes -- Exercise 1C: Using Common Laboratory Tools to Evaluate Measurements Post-laboratory Thinking Questions -- Directions -- Exercise 2A: Using Microscopy to Evaluate Cell Size and Complexity Pre-laboratory Thinking Questions -- Directions -- Exercise 2B: Using Microscopy to Evaluate Cell Size and Complexity -- Microscopy Introduction -- Let's Put This to Practice! -- Procedure: Steps Used When Viewing a Slide -- Exercise 2C: Using Microscopy to Evaluate Cell Size and Complexity Post-laboratory Thinking Questions -- Directions -- Exercise 3A: The Bacterial Growth Curve Pre-laboratory Thinking Questions -- Directions -- Exercise 3B: The Bacterial Growth Curve -- Introduction -- Let's Put This to Practice! -- Sample Problems for Graphing on Excel -- Answer to Excel Sample Problems -- Exercise 3C: The Bacterial Growth Curve Post-laboratory Thinking Questions -- Directions -- Module 2 Working with and Learning About Serratia marcescens in the Laboratory -- Exercise 4A: Protein Concentration Versus Growth Stage Pre-laboratory Thinking Questions -- Directions -- Exercise 4B: Protein Concentration Versus Growth Stage -- Introduction -- Let's Put This to Practice! -- Lysis Protocol -- Lowry Assay -- Exercise 4C: Protein Concentration Versus Growth Stage Post-laboratory Thinking Questions |
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Directions -- Exercise 5A: Measuring Prodigiosin Pre-laboratory Thinking Questions -- Directions -- Exercise 5B: Measuring Prodigiosin -- Introduction -- Let's Put This to Practice! -- Exercise 5C: Measuring Prodigiosin Post-laboratory Thinking Questions -- Directions -- Exercise 6A: Conditions Affecting the Growth of and Prodigiosin Production by Serratia marcescens Pre-laboratory Thinking Questions -- Directions -- Exercise 6B: Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens -- Introduction -- Let's Put This to Practice! -- Exercise 6C: Formal Laboratory Report Describing the Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens -- Introduction -- General Instructions -- Exercise 7A: Biochemistry of Prodigiosin Production Pre-laboratory Thinking Questions -- Directions -- Exercise 7B: Biochemistry of Prodigiosin Production -- Introduction -- Let's Put This to Practice! -- Exercise 7C: Biochemistry of Prodigiosin Production Post-laboratory Thinking Questions -- Directions -- Exercise 8A: The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise Pre-laboratory Thinking Questions -- Exercise 8B: The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise -- Introduction -- Let's Put This to Practice! -- Exercise 8C: The Probability Basis for Mutation Rate Calculation: A Dice-Roll Exercise Post-laboratory Thinking Questions -- Exercise 9A: Understanding Evolution by the Generation of UV Light-Induced Prodigiosin Mutants Pre-laboratory Thinking Questions -- Directions -- Exercise 9B: Understanding Evolution by the Generation of UV Light-Induced Prodigiosin Mutants -- Introduction -- Let's Put This to Practice! -- Exercise 9C: Understanding Evolution by the Generation of UV Light-Induced Prodigiosin Mutants Post-laboratory Thinking Questions -- Directions |
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Exercise 10A: Understanding the Energy Spilling Properties of Prodigiosin Pre-laboratory Thinking Questions -- Directions -- Exercise 10B: Understanding the Energy Spilling Properties of Prodigiosin -- Introduction -- Let's Put This to Practice! -- Exercise 10C: Understanding the Energy Spilling Properties of Prodigiosin Post-laboratory Thinking Questions -- Directions -- Module 3 Initial Characterization of Novel Serratia marcescens Prodigiosin Mutants -- Exercise 11A: Prodigiosin Mutant Study Part 1 Pre-laboratory Thinking Questions -- Directions -- Exercise 11B: Prodigiosin Mutant Study Part 1 -- Introduction -- Let's Put This to Practice! -- Exercise 11C: Prodigiosin Mutant Study Part 1 Post-laboratory Thinking Questions -- Directions -- Exercise 12A: Prodigiosin Mutant Study Part 2 Pre-laboratory Thinking Questions -- Directions -- Exercise 12B: Prodigiosin Mutant Study Part 2 -- Introduction -- Let's Put This to Practice! -- Exercise 12C: Formal Laboratory Report 2: Prodigiosin Mutant Study -- General Instructions -- Appendix A CSE Citation and Reference List Format Guidelines -- Appendix B Prodigiosin Biosynthesis -- References -- Index -- Supplemental Images -- EULA |
| Summary |
The Fundamentals of Scientific Research: An Introductory Laboratory Manual is a laboratory manual geared towards first semester undergraduates enrolled in general biology courses focusing on cell biology. This laboratory curriculum centers on studying a single organism throughout the entire semester - Serratia marcescens, or S. marcescens, a bacterium unique in its production of the red pigment prodigiosin. The manual separates the laboratory course into two separate modules. The first module familiarizes students with the organism and lab equipment by performing growth curves, Lowry protein assays, quantifying prodigiosin and ATP production, and by performing complementation studies to understand the biochemical pathway responsible for prodigiosin production. Students learn to use Microsoft Excel to prepare and present data in graphical format, and how to calculate their data into meaningful numbers that can be compared across experiments. The second module requires that the students employ UV mutagenesis to generate hyper-pigmented mutants of S. marcescens for further characterization. Students use experimental data and protocols learned in the first module to help them develop their own hypotheses, experimental protocols, and to analyze their own data. Before each lab, students are required to answer questions designed to probe their understanding of required pre-laboratory reading materials. Questions also guide the students through the development of hypotheses and predictions. Following each laboratory, students then answer a series of post-laboratory questions to guide them through the presentation and analysis of their data, and how to place their data into the context of primary literature. Students are also asked to review their initial hypotheses and predictions to determine if their conclusions are supportive. A formal |
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Laboratory report is also to be completed after each module, in a format similar to that of primary scientific literature. The Fundamentals of Scientific Research: An Introductory Laboratory Manual is an invaluable resource to undergraduates majoring in the life sciences |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (EBSCO, viewed August 26, 2015) |
| Subject |
Laboratories -- Handbooks, manuals, etc
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Research -- Handbooks, manuals, etc
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SCIENCE -- Study & Teaching.
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Laboratories
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Research
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| Genre/Form |
Handbooks and manuals
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| Form |
Electronic book
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| Author |
Haddix, Pryce L., contributor
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| ISBN |
9781118867884 |
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1118867882 |
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9781118867723 |
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1118867726 |
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