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Author Sironi, Laura, 1982- author

Title Optical blood flow measurement in microcirculatory systems / by Laura Sironi, Laura D'Alfonso, Margaux Bouzin, Nicolo Giovanni Ceffa, Maddalena Collini, Giuseppe Chirico, and Pablo Blinder
Published Bellingham, Washington (1000 20th St. Bellingham WA 98225-6705 USA) : SPIE, 2016
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Description 1 online resource (iv, 94 pages)
Series SPIE. Spotlight ; SL23
SPIE spotlight ; SL23
Contents 1. Introduction -- 2. Laser speckle contrast imaging: 2.1. Principles; 2.2. Instrument configuration and data acquisition; 2.3. Applications -- 3. Doppler optical coherence tomography: 3.1. Principles; 3.2. Instrument configuration and data acquisition; 3.3. Applications -- 4. Diffuse correlation spectroscopy: 4.1. Principles; 4.2. Instrument configuration and data acquisition; 4.3. Applications -- 5. Multiplexed and image correlation methods: video-based acquisition: 5.1. Dual focus cross-correlation; 5.2. Particle image velocimetry; 5.3. Spatiotemporal image correlation spectroscopy; 5.4. Applications; 5.5. Other related methods -- 6. Space-time diagrams and image correlation methods: scanning-based acquisition and detection: 6.1. Line-scan and arbitrary path blood flow measurements; 6.2. Flow image correlation spectroscopy; 6.3. Instrument configuration and data acquisition; 6.4. Applications -- References
Summary This Spotlight reviews non-invasive optical methods to determine the blood flow velocity in microcirculation, with an emphasis on the most recent developments that may be routinely applied in cellular biology laboratories. The methods covered in this book include both scanning and whole-field optical imaging techniques based on endogenous or exogenous (fluorescent) contrast agents for plasma and red-blood-cell visualization, such as laser Doppler velocimetry, laser speckle contrast imaging, diffuse correlation spectroscopy, particle image velocimetry, and two-photon microscopy. Several methods have been developed to quantify the blood flow velocity within microvessels by exploiting the cross-correlation concept applied to optical scanning microscopes: dual-foci cross-correlation, spatio-temporal image correlation spectroscopy, line-scanning techniques, and a recently developed single raster-scanned xy-image technique (FLICS). The basic operational principles, the advantages and disadvantages, and the technical issues of these methods will be discussed and compared, and their applications in biomedical research will be presented
Bibliography Includes bibliographical references (pages 64-94)
Notes Title from PDF title page (SPIE eBooks Website, viewed 2016-11-11)
Subject Blood flow -- Measurement.
Laser Doppler blood flowmetry.
Microcirculation.
Blood Flow Velocity.
Microcirculation.
Laser-Doppler Flowmetry.
Form Electronic book
Author Blinder, Pablo, 1973- author
Bouzin, Margaux, author
Ceffa, Nicolò Giovanni, author
Chirico, Giuseppe, 1963- author
Collini, Maddalena, 1964- author
D'Alfonso, Laura, 1972- author
Society of Photo-optical Instrumentation Engineers, publisher
ISBN 1510605002 (electronic bk.)
9781510605008 (electronic bk.)