| Description |
1 online resource (xiii, 613 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1940-6029 ; Volume No. 1200 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1200. 1064-3745
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| Contents |
PART I. STANDARD TECHNIQUES -- 1. History of Plant Lectin Research / Els J.M. Van Damme -- 2. Fungal Lectins: A Growing Family / Yuka Kobayashi and Hirokazu Kawagishi -- 3. The "White Kidney Bean Incident" in Japan / Haruko Ogawa and Kimie Date -- 4. Hemagglutination (Inhibition) Assay / Kotone Sano and Haruko Ogawa -- 5. Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources / Kimie Date and Haruko Ogawa -- 6. High-Performance Lectin Affinity Chromatography / Yuka Kobayashi -- 7. Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography / Katsuko Yamashita and Takashi Ohkura -- 8. Lectin-Probed Western Blot Analysis / Takeshi Sato -- 9. Solid-Phase Assay of Lectin Activity Using HRP-Conjugated Glycoproteins / Kyoko Kojima-Aikawa -- 10. A Simple Viral Neuraminidase-Based Detection for High- Throughput Screening of Viral Hemagglutinin-Host Receptor Specificity / Nongluk Sriwilaijaroen and Yasuo Suzuki -- 11. Lectin Affinity Electrophoresis / Yuka Kobayashi -- 12. Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis Between Lectins and Glycans / Mitsuhiro Kinoshita and Kazuaki Kakehi -- 13. Basic Procedures for Lectin Flow Cytometry / Kenta Moriwaki and Eiji Miyoshi -- 14. Histochemical Staining Using Lectin Probes / Yoshihiro Akimoto and Hayato Kawakami -- 15. Equilibrium Dialysis Using Chromophoric Sugar Derivatives / Tomomitsu Hatakeyama -- 16. Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis Between Lectins and Sugars / Kanji Hori and Makoto Hirayama -- 17. Surface Plasmon Resonance as a Tool to Characterize Lectin-Carbohydrate Interactions / Yasuro Shinohara and Jun-ichi Furukawa -- 18. Isothermal Calorimetric Analysis of Lectin-Sugar Interaction / Yoichi Takeda and Ichiro Matsuo -- 19. Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines / Mamoru Mizuno |
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PART II. EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS -- 20. Lectin-Based Glycomics: How and When Was the Technology Born? / Jun Hirabayashi -- 21. Frontal Affinity Chromatography (FAC): Theory and Basic Aspects / Ken-ichi Kasai -- 22. Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides / Chihiro Sato -- 23. Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation--From Sample Pretreatment to Data Standardization / Atsushi Kuno, Atsushi Matsuda, Sachiko Unno, Binbin Tan, Jun Hirabayashi, and Hisashi Narimatsu -- 24. Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces / Azusa Shibazaki and Tohru Gonoi -- 25. Application of Lectin Microarray to Bacteria Including Lactobacillus casei/paracasei Strains / Emi Yasuda, Tomoyuki Sako, Hiroaki Tateno, and Jun Hirabayashi -- 26. Live-Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN / Hiroaki Tateno, Yasuko Onuma, and Yuzuru Ito -- 27. Carbohydrate-Binding Specificity of Lectins Using Multiplexed Glyco-Bead Array / Kazuo Yamamoto -- 28. Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins / Yu-ki Matsuno and Akihiko Kameyama |
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PART III. TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS -- 29. Overall Strategy for Functional Analysis of Animal Lectins / Norihito Kawasaki -- 30. Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System / Hiroaki Tateno -- 31. Potential Usage for In Vivo Lectin Screening in Live Animals Utilizing Cell Surface Mimetic Glyco- nanoparticles, Phosphorylcholine-Coated Quantum Dots (PC-QDs) / Maho Amano, Hiroshi Hinou, Risho Miyoshi, and Shin-Ichiro Nishimura -- 32. Remodeling Cell Surface Glycans Using Glycosylation Inhibitors / Norihito Kawasaki -- 33. Remodeling of Glycans Using Glycosyltransferase Genes / Yuko Naito-Matsui and Hiromu Takematsu -- 34. Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression) / Motohiro Nonaka and Toshisuke Kawasaki -- 35. Analysis of L-Selectin-Mediated Cellular Interactions Under Flow Conditions / Hiroto Kawashima -- 36. Assessment of Weak Sugar-Binding Ability Using Lectin Tetramer and Membrane-Based Glycans / Kazuo Yamamoto |
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PART IV. STRUCTURAL BIOLOGY AND ENGINEERING OF LECTINS -- 37. Perspectives in Glycomics and Lectin Engineering / Jan Tkac, Tomas Bertok, Jozef Nahalka, and Peter Gemeiner -- 38. Molecular Basis of a Pandemic of Avian-Type Influenza Virus / Nongluk Sriwilaijaroen and Yasuo Suzuki -- 39. Basic Procedure of X-Ray Crystallography for Analysis of Lectin-Sugar Interactions / Zui Fujimoto -- 40. A New Structure Determination Method of Lectins Using a Selenium-Containing Sugar Ligand / Hisayoshi Makyio and Ryuichi Kato -- 41. NMR Analysis of Carbohydrate-Binding Interactions in Solution: An Approach Using Analysis of Saturation Transfer Difference NMR Spectroscopy / Hikaru Hemmi -- 42. Small-Angle X-Ray Scattering to Obtain Models of Multivalent Lectin-Glycan Complexes / Stephen D. Weeks and Julie Bouckaert -- 43. Directed Evolution of Lectins by an Improved Error-Prone PCR and Ribosome Display Method / Dan Hu, Hiroaki Tateno, and Jun Hirabayashi -- 44. Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering / Tomohisa Ogawa and Tsuyoshi Shirai |
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PART V. COMPREHENSIVE LISTS -- 45. Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities / Yuka Kobayashi, Hiroaki Tateno, Haruko Ogawa, Kazuo Yamamoto, and Jun Hirabayashi -- 46. Lectin Structures: Classification Based on the 3-D Structures / Zui Fujimoto, Hiroaki Tateno, and Jun Hirabayashi |
| Summary |
Annotation Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on "engineer" novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists |
| Analysis |
lectinen |
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lectins |
|
eiwitten |
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proteins |
|
eiwitexpressieanalyse |
|
proteomics |
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Proteins and Enzymes |
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Eiwitten en enzymen |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Online resource; title from PDF title page (SpringerLink, viewed August 27, 2014) |
| Subject |
Lectins -- Laboratory manuals
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Lectins -- genetics
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Glycomics -- methods
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Lectins
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Biology -- Laboratory manuals
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Hirabayashi, Jun, editor.
|
| ISBN |
9781493912926 |
|
1493912925 |
|
1493912917 |
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9781493912919 |
|
