Limit search to available items
Book Cover
E-book
Author Svergun, D. I. (Dmitriĭ Ivanovich), author.

Title Small angle x-ray and neutron scattering from solutions of biological macromolecules / Dmitri I. Svergun, Michael H.J. Koch, Peter A. Timmins, Roland P. May
Edition First edition
Published Oxford : Oxford University Press, 2013

Copies

Description 1 online resource (ix, 358 pages) : illustrations (black and white
Series International Union of Crystallography monographs on crystallography ; 19
Oxford science publications
International Union of Crystallography monographs on crystallography ; 19.
Oxford science publications.
Contents ""Cover""; ""Contents""; ""Introduction""; ""References""; ""PART I: THEORY AND EXPERIMENT""; ""1 Basics of small angle scattering""; ""1.1 Elastic scattering of X-rays and neutrons""; ""1.2 Scattering by macromolecular solutions""; ""1.3 Resolution and contrast""; ""1.4 Absorption and anomalous X-ray scattering""; ""References""; ""2 X-ray and neutron scattering instruments""; ""2.1 Characteristics of sources""; ""2.2 X-ray sources""; ""2.3 Neutron sources""; ""2.4 SAXS instruments""; ""2.5 SANS instruments""; ""2.6 Special instruments""; ""References""
""3 Experimental practice and data processing""""3.1 Sample requirements for SAXS and SANS""; ""3.2 Experimental protocols and instrumental corrections""; ""3.3 Special features of SAXS: radiation damage""; ""3.4 Special features of SANS: the use of H[Sub(2)]O/D[Sub(2)]O mixtures""; ""3.5 Random and systematic errors""; ""3.6 Basic structural information and data quality""; ""3.7 Calibration to absolute scale and molecular mass""; ""References""; ""PART II: DATA ANALYSIS METHODS""; ""4 Monodisperse systems""; ""4.1 Overall parameters of particles""
""4.2 Multipole representation of SAS intensity""""4.3 Shannon sampling""; ""4.4 Ab initio shape analysis""; ""4.5 Computation of scattering patterns from atomic models""; ""4.6 Hybrid methods""; ""4.7 Labelling and triangulation""; ""References""; ""5 Polydisperse and interacting systems""; ""5.1 Size, shape and conformation polydispersity""; ""5.2 Size distribution functions""; ""5.3 Shape polydispersity and oligomeric mixtures""; ""5.4 Conformational polydispersity and flexible systems""; ""5.5 Interacting systems and structure factor""; ""References""
""PART III: BIOLOGICAL APPLICATIONS OF SOLUTION SAS""""6 Static structural studies""; ""6.1 Applications of ab initio shape determination""; ""6.2 Quaternary structure analysis of proteins and complexes""; ""6.3 Equilibrium mixtures and oligomeric composition""; ""6.4 Membrane proteins and lipoproteins""; ""6.5 Flexible systems""; ""References""; ""7 Kinetic and perturbation studies""; ""7.1 Dynamics and kinetics""; ""7.2 Perturbation methods""; ""7.3 Temperature scans and T-jumps""; ""7.4 High-pressure experiments""; ""7.5 Stopped-flow and continuous-flow mixing""
""7.6 Light-triggered processes""""References""; ""8 Analysis of interparticle interactions""; ""8.1 Basic physical chemistry of interactions""; ""8.2 Experimental SAS studies of protein�protein interactions""; ""8.3 Structure factor calculations for proteins""; ""8.4 Interactions in nucleic acids""; ""References""; ""9 SAS in multidisciplinary studies""; ""9.1 Automation and high-throughput SAS""; ""9.2 Joint use with high-resolution methods""; ""9.3 SAS and low-resolution crystallography""; ""9.4 Complementary biophysical methods""; ""9.5 Bioinformatics and model validation""
Summary Describing all aspects of the technique of small-angle scattering of X-rays and neutrons, this book covers topics including instrumentation, sample requirements, data interpretation and modelling methods. Examining each in a comprehensive way, the text also gives examples of applications in various fields of biophysics and biochemistry
Bibliography Includes bibliographical references and index
Notes Print version record
Subject X-rays -- Scattering.
Small-angle scattering.
Macromolecules -- Structure
Macromolecules -- Measurement
Neutrons -- Scattering.
x-ray scattering.
SCIENCE / Life Sciences / Biochemistry.
Macromolecules -- Measurement
Macromolecules -- Structure
Neutrons -- Scattering
Small-angle scattering
X-rays -- Scattering
Form Electronic book
Author Koch, Michel H. J., author.
Timmins, Peter A. (Scientist), author.
May, Roland P., author.
ISBN 0191507032
9780191507038
9780191747731
0191747734