| Description |
1 online resource (220 pages) |
| Contents |
Preface; 1 Choice of adequate sample material; 1.1 Viruses; 1.2 Bacteria; 1.3 Fungi; 1.4 Protozoa; 2 Stability of the specimen during preanalytics; 2.1 Degradation of DNA; 2.2 Degradation of RNA; 2.3 Inhibitors of PCR; 2.4 How can contamination during specimen collection and in the laboratory be avoided?; 2.5 How can the sample identity be ensured?; 2.6 Transport of diagnostic material; 2.6.1 Category A Infectious Substances; 2.6.2 Category B Infectious Substances; 2.6.3 Exempt patient specimens; 2.7 Stability of nucleic acids of selected pathogens during preanalytics |
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2.7.1 Human immunodeficiency virus type 1 (HIV-1) RNA2.7.2 Hepatitis B virus (HBV) DNA; 2.7.3 Hepatitis C virus (HCV) RNA; 2.7.4 Chlamydia trachomatis and Neisseria gonorrhoeae DNAs; 2.7.5 Viral pathogens producing respiratory tract infections; 2.7.6 Pathogens in stool specimens; 2.8 Take-home messages; 2.9 Further reading; 3 Quality assurance and quality control; 3.1 Accreditation issues; 3.2 Validation and verification work; 3.3 Components of validation work; 3.3.1 Internal and external quality controls; 3.3.2 Proficiency testing; 3.3.3 Validation of employee competency |
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3.3.4 Instrument maintenance and calibration3.3.5 Correlation with clinical findings; 3.4 Components of verification work; 3.4.1 Components of verification work for IVD/CE labeled and/or FDA-approved or -cleared tests or test systems; 3.4.2 Components of verification work for laboratory-developed tests or test systems; 3.5 Take-home messages; 3.6 Further reading; 4 Extraction of nucleic acids; 4.1 Manual nucleic acid extraction protocols; 4.2 Automated nucleic acid extraction platforms; 4.2.1 Technology principle; 4.2.2 Desirable features of automated platforms |
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4.3 Preparation of PCR mixes and addition of eluates4.4 Currently frequently used commercially available platforms for nucleic acid extraction; 4.5 Take-home messages; 4.6 Further reading; 5 Amplification and detection methods; 5.1 Nucleic acid-based tests; 5.2 Target amplification methods; 5.2.1 Real-time polymerase chain reaction (qPCR); 5.2.2 Isothermal amplification techniques; 5.2.3 Next generation sequencing (NGS); 5.3 Signal amplification methods; 5.3.1 Branched DNA (bDNA); 5.3.2 Hybrid capture assay; 5.4 What are the key challenges for the future?; 5.5 Take-home messages |
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5.6 Further reading6 Interpreting and reporting molecular diagnostic tests; 6.1 Qualitative and quantitative detection of viral infections; 6.2 Detection of bacterial infections; 6.3 Quantitative endpoint PCR; 6.4 Real time PCR; 6.5 Reporting results; 6.5.1 Genetic names; 6.5.2 Recommendations for reporting results of molecular tests; 6.5.3 Recommendations for the contents of the molecular test report; 6.6 Interpretation; 6.7 Important issues when clinically interpreting molecular diagnostic results; 6.8 Take-home messages; 6.9 Further reading; 7 Human immunodeficiency virus |
| Summary |
The prevalence of infectious diseases is worldwide increasing. Therefore, detection methods for infectious pathogens change quickly. In the new edition of Kesslerś Molecular Diagnostics of Infectious Diseases laboratory professionalists get valueable information about the current diagnostic methods, tipps and tricks in terms of sample processing, quality control, and interpretation of the results. For clinicians the book is a valuable aid for decision-making in ordering appropriate tests as well as in assuring the necessary quality of the sample material |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Communicable diseases -- Molecular diagnosis
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Communicable diseases.
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Medical laboratory technology.
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Genetics -- Technique.
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Infection.
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Diseases.
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Molecular Diagnostic Techniques
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Communicable Diseases
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Clinical Laboratory Techniques
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Genetic Techniques
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Infections
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Bacterial Infections and Mycoses
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Investigative Techniques
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Disease
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Communicable diseases
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Diseases
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Genetics -- Technique
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Infection
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Medical laboratory technology
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| Form |
Electronic book
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| ISBN |
9783110278934 |
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3110278936 |
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3110278839 |
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9783110278835 |
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1283857898 |
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9781283857895 |
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3110278928 |
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9783110278927 |
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