| Description |
1 online resource (xvi, 461 pages) : illustrations (some color) |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; 597 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 597
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| Contents |
The Rat: a model used in biomedical research / Howard J. Jacob -- Genetic mapping and positional cloning / Timothy J. Aitman, Enrico Petretto, Jacques Behmoaras -- Sequencing of the rat genome and databases / Elizabeth A. Worthey, Alexander J. Stoddard, Howard J. Jacob -- Design of expression cassettes for the generation of transgenic animals (including insulators) / Louis-Marie Houdebine -- Inducible and conditional promoter systems to generate transgenic animals / Yoji Hakamata, Eiji Kobayashi -- Generation of transgenic rats by microinjection of short DNA fragments / Séverine Ménoret, Séverine Remy, Claire Usal, Laurent Tesson, Ignacio Anegon -- Generation of transgenic rats using YAC and BAC DNA constructs / Ri-ichi Takahashi, Masatsugu Ueda -- The Use of lentiviral vectors to obtain transgenic rats / Séverine Remy, Tuan Huy Nguyen, Séverine Ménoret, Laurent Tesson, Claire Usal, Ignacio Anegon -- Generation of transgenic rats by ooplasmic injection of sperm cells exposed to exogenous DNA / Masumi Hirabayashi, Shinichi Hochi -- Procedures for somatic cell nuclear transfer in the rat / Jean Cozzi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Yacine Cherifi, Qi Zhou -- ENU mutagenesis to generate genetically modified rat models / Ruben Boxtel, Michael N. Gould, Edwin Cuppen, Bart M.G. Smits -- Establishment of embryonic stem cells from rat blastocysts / Masaki Kawamata, Takahiro Ochiya -- Derivation, culture, and in vivo developmental capacity of embryonic cell lines from rat blastocysts / Simon-Pierre Demers, Lawrence C. Smith -- Interference RNA for in vivo knock-down of gene expression or genome-wide screening using shRNA / Silvère Petit, Kader Thiam -- Generation of gene-specific mutated rats using zinc-finger nucleases / Aron M. Geurts, Gregory J. Cost, Séverine Rémy, Xiaoxia Cui, Laurent Tesson, Claire Usal, Séverine Ménoret, Howard J. Jacob, Ignacio Anegon, Roland Buelow -- Application of microarray-based analysis of gene expression in the field of toxicogenomics / Nan Mei, James C. Fuscoe, Edward K. Lobenhofer, Lei Guo -- Generation of congenic and consomic rat strains / Dominique Lagrange, Gilbert J Fournié -- Generation of rat "supersonic" congenic/conplastic strains using superovulation and embryo transfer / Vladimír Landa, Václav Zídek, Michal Pravenec -- Analysis by quantitative PCR of zygosity in genetically modified organisms / Laurent Tesson, Séverine Rémy, Séverine Ménoret, Claire Usal, Ignacio Anegon -- A Restriction enzyme-PCR-based technique to determine transgene insertion sites / Elizabeth C. Bryda, Beth A. Bauer -- Cryopreservation and orthotopic transplantation of rat ovaries / Martina Dorsch, Dirk Wedekind -- Techniques for in vitro and in vivo fertilization in the rat / Naomi Kashiwazaki, Yasunari Seita, Akiko Takizawa, Naoki Maedomari, Junya Ito, Tadao Serikawa -- Rat strain repositories / Birger Voigt -- Neurobehavioral tests in rat models of degenerative brain diseases / Yvonne K. Urbach, Felix J. Bode, Huu Phuc Nguyen, Olaf Riess, Stephan von Hörsten -- Rat genomics applied to psychiatric research / Marie-Pierre Moisan, André Ramos -- Genomics studies of immune-mediated diseases using the BN-LEW rat model / Isabelle Bernard, Gilbert J. Fournié, Abdelhadi Saoudi -- Rat models of cardiovascular diseases / Michael Bader -- Use of rat genomics for investigating the metabolic Syndrome / Michal Pravenec -- Genomic research in rat models of kidney disease / Yoram Yagil, Chana Yagil -- Cancer research in rat models / Claude Szpirer |
| Summary |
Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems. The volume extensively covers topics including genome sequencing, quantitative trait loci mapping, and the identification of single nucleotide polymorphisms as well as the development of transgenic technologies such as nuclear cloning, lentiviral-mediated transgenesis, gene knock-down using RNA interference, gene knock-out by mutagenesis, and zinc finger nucleases plus exciting advances in the obtention of rat embryonic cell lines. As a volume in the highly successful Methods in Molecular Biology series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Comprehensive and up-to-date, Rat Genomics: Methods and Protocols thoroughly covers the current techniques used in labs around the world and overviews the applications of the data obtained, making it certain to be useful to the scientific community as a key source of references and methods |
| Analysis |
ratten |
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rats |
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transgene dieren |
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transgenic animals |
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genexpressieanalyse |
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genomics |
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Laboratory Animals |
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Genome informatics |
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Laboratoriumdieren |
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Genoominformatica |
| Notes |
Title from PDF title page (SpringerLink, viewed January 6, 2010) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Rats -- Molecular genetics
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Genomics.
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Rats -- Functional genomics
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Genomics
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Rats -- genetics
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Genomics
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Anegon, Ignacio
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| ISBN |
9781603273893 |
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1603273891 |
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