| Description |
1 online resource (xiii, 306 pages) : illustrations |
| Series |
Methods in molecular medicine ; 113 |
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Methods in molecular medicine ; 113.
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| Contents |
Multiple myeloma : challenges and opportunities / Douglas Joshua, Ross Brown, and P. Joy Ho -- Immunophenotyping of plasma cells in multiple myeloma / Gema Mateo Manzanera, Jesus F. San Miguel Izquierdo, and Alberto Orfao de Matos -- Plasma cell labeling index / Philip R. Griepp and Shaji Kumar -- Conventional cytogenetics in myeloma / Lynda J. Campbell -- Multicolour spectral karyotyping in multiple myeloma / Jeffrey R. Sawyer -- Detection of chromosome 13 deletions by fluorescent in situ hybridization / Scott van Wier and Rafael Fonseca -- Comparative genomic hybridization for analysis of changes in DNA copy number changes in multiple myeloma / Pulivarthi H. Rao -- Southern blotting of IgH rearrangements in B cell disorders / Edna Yuen and Ross D. Brown -- Identification and assembly of V gene as idiotype-specific DNA fusion vaccines in multiple myeloma / Surinder S. Sahota, Mark Townsend, and Freda K. Stevenson -- Identification of clonotypic IgH VDJ sequences in multiple myeloma / Brian J. Taylor [and others] -- Real-time Polymerase chain reaction of immunoglobulin rearrangements for quantitative evaluation of minimal residual disease in myeloma / Mara Compagno [and others] -- Incomplete DJH rearrangements / David Gonzalez and Ramon Garcia-Sanz .. |
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Identification of malignant plasma cells by mRNA in situ hybridization / Ross D. Brown and P. Joy Ho -- The SCID-hu myeloma model / Joshua Epstein and Shmuel Yaccoby -- The 5T2MM murine model of multiple myeloma : maintenance and analysis / Karin Vanderkerken [and others] -- Determination of telomerase activity and telomere length / Kai-da Wu and Malcolm A.S. Moore -- Dendritic cell/myeloma hybrid vaccine / Dajing Xia, Tim Chan, and Jim Xiang -- Genetically engineered myeloma cell vaccine / Siguo Hao, Tim Chan, and Jim Xiang -- An in vitro osteoclast-forming assay to measure myeloma cell-derived osteoclast-activating factors / Andrew C.W. Zannettino [and others] -- Clonality detection of the expanded T-cell populations in patients with multiple myeloma / Daniel M.-Y. Sze -- Detecting mismatch repair defects in myeloma / Mark Velangi [and others] -- Methylation-specific polymerase chain reaction / Oliver Galm and James G. Herman -- Making sense of DNA microarray data / Wei-min Liu, Sunhee K. Ro, and Walter H. Koch |
| Summary |
Although there is no therapy that could be considered curative for patients with multiple myeloma, recent research has gained considerable momentum and yielded a real prospect for achieving a significant improvement in response to therapy. In Multiple Myeloma: Methods and Protocols, established researchers with hands-on experience detail a comprehensive set of classic and innovative methodologies used in many laboratories for the investigation of this disease. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology, which is used routinely in many laboratories around the world to determine prognosis, to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Multiple Myeloma: Methods and Protocols offers both novice and experienced researchers alike a gold-standard collection of proven techniques for investigating the pathogenesis of multiple myeloma and identifying new therapeutic targets |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Multiple myeloma -- Molecular aspects -- Laboratory manuals
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Multiple Myeloma -- immunology
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Multiple Myeloma -- genetics
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MEDICAL -- Oncology.
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HEALTH & FITNESS -- Diseases -- Cancer.
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Molekulare Medizin
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Plasmozytom
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| Genre/Form |
Laboratory manuals.
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Brown, Ross D.
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Ho, P. Joy.
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| ISBN |
1588293920 |
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9781588293923 |
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9781592599165 |
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1592599168 |
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1280359110 |
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9781280359118 |
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9786610359110 |
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6610359113 |
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