Title Mycoplasma protocols / edited by Roger Miles and Robin Nicholas

Published Totowa, N.J. : Humana Press, 1998

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Description 1 online resource (xiii, 330 pages) Series Methods in molecular biology ; v. 104 Methods in molecular biology (Clifton, N.J.) ; v. 104 Contents Medical significance of mycoplasmas / Paul Taylor -- The veterinary significance of mycoplasmas / Robin Nicholas -- Recovery of human mycoplasmas / Paul Taylor -- Recovery of mycoplasmas from animals / Robin Nicholas, Samantha Baker -- Recovery of mycoplasmas from birds / Janet M. Bradbury -- Cultivation of ureaplasmas / Patricia M. Furr -- Quality-control testing of mycoplasma medium / David Windsor, Helena Windsor -- Biochemical characteristics in mycoplasma identification / José B. Poveda -- Enzyme analysis : the rationale and use of enzyme assays in assigning function to gene nucleotide sequences and the procedures for the assay of three enzymatic functions conserved in mollicutes / J. Dennis Pollack -- Determination of substrate utilization rates by mycoplasmas / Roger J. Miles, Calista Agbanyim -- Serological identification of mycoplasmas by growth and metabolic inhibition tests / José B. Poveda, Robin Nicholas -- Identification of mycoplasmas by dot immunobinding on membrane filtration (MF Dot) / Francois Poumarat -- Identification of mycoplasmas by immunofluorescence / Janet M. Bradbury -- Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs / Hywel J. Ball, David Finlay -- Immunohistochemical staining of fixed tissues / Eugenio Scanziani -- Extraction of DNA from mycoplasmas / John B. Bashiruddin -- Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers / Karl-Erik Johansson, Malin U. Heldtander, Bertil Pettersson -- PCR and RFLP methods for the specific detection and identification of Mycoplasma mycoides subsp. mycoides SC / John B. Bashiruddin -- Characterization of mycoplasmas by RAPD fingerprinting / Georges A. Rawadi -- Classification of isolates by DNA-DNA hybridization / Konrad Sachse, Helmut Hotzel -- Insertion sequence analysis / Joachim Frey -- Detection of mycoplasmas in cell cultures by cultural methods / Gerald K. Masover, Frances A. Becker -- Detection of mycoplasmas in cell cultures by fluorescence methods / Gerald K. Masover, Frances A. Becker -- Transformation of mycoplasmas / Chris F. Minion, Paul A. Kapke -- Transposon mutagenesis / LeRoy L. Voelker, Kevin Dybvig -- Demonstration of extrachromosomal elements / LeRoy L. Voelker, Kevin Dybvig -- Expression of foreign genes in Acholeplasma laidlawii / Tanja K Jarhede, Åke Wieslander -- Mycoplasma gene expression in Escherichia coli / Chris F. Minion -- Polyacrylamide gel-electrophoresis separation of whole-cell proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham -- Immunological and biochemical characterization of membrane proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham -- Detection and analysis of mycoplasma adhesins / Konrad Sachse -- Transmission electron microscopy and immunogold staining of mollicute surface antigens / Gunna Christiansen, Svend Birkelund Summary In Mycoplasma Protocols, Roger Miles and Robin Nicholas present a collection of cutting-edge methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenicity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products. Mycoplasma Protocols provides up-to-date and easy-to-follow mycoplasma methods for practical application in medical and veterinary diagnostic and research laboratories. The techniques permit effective work with these normally fastidious microorganisms, allowing investigators to illuminate their roles across a wide range of chronic respiratory, arthritic, and urogenital diseases. For all those working in this important field, Mycoplasma Protocols immediately becomes the methodological resource of choice Analysis mycoplasma humane ziekten human diseases dierziekten animal diseases biologische technieken biological techniques detectie detection identificatie identification karakterisering characterization methodologie methodology protocollen protocols Medicine (General) Bacteriology Bacteria Infectious Diseases Geneeskunde (algemeen) Bacteriologie Infectieziekten Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Mycoplasma -- Laboratory manuals Mycoplasma diseases -- Laboratory manuals Mycoplasma SCIENCE -- Life Sciences -- Biology. SCIENCE -- Life Sciences -- Microbiology. Mycoplasma Mycoplasma diseases Mycoplasma's. Wetenschappelijke technieken. Genre/Form Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Miles, Roger (Roger J.) Nicholas, Robin, 1951- LC no. 98004288 ISBN 9781592592692 0585328552 9780585328553 1592592694 0896035255 9780896035256

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