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Title Nanomaterial interfaces in biology : methods and protocols / edited by Paolo Bergese, Kimberly Hamad-Schifferli
Published New York : Humana Press, [2013]
©2013

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Description 1 online resource (xi, 293 pages) : illustrations (some color)
Series Methods in Molecular Biology ; 1025
Methods in molecular biology (Clifton, N.J.) ; v. 1025. 1064-3745
Contents Preparation of 2 nm gold nanoparticles for in vitro and in vivo applications / Daniel F. Moyano, Bradley Duncan, and Vincent M. Rotello -- DNA conjugation to nanoparticles / Sunho Park -- Conjugation of nanoparticles to proteins / Marie-Eve Aubin-Tam -- Water-solubilization and functionalization of semiconductor quantum dots / Christina M. Tyrakowski, Adela Isovic, and Preston T. Snee -- Synthesizing and modifying peptides for chemoselective ligation and assembly into quantum dot-peptide bioconjugates / W. Russ Algar [and others] -- Reliable methods for silica coating of au nanoparticles / Isabel Pastoriza-Santos and Luis M. Liz-Marzán -- Surface modifications by polymers for biomolecule conjugation / Laura Sola [and others] -- Functionalization protocols of silicon micro/nano- mechanical biosensors / Francesca Frascella and Carlo Ricciardi -- Stability and aggregation assays of nanoparticles in biological media / James Chen Yong Kah -- Electrochemical measurements of DNA melting on surfaces / Irina Belozerova, Dongbiao Ge, and Rastislav Levicky -- Formation and characterization of the nanoparticle-protein corona / Marco P. Monopoli [and others] -- Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution / Hyeran Noh [and others] -- Hyperspectral microscopy for characterization of gold nanoparticles in biological media and cells for toxicity assessment / Christin Grabinski, John Schlager, and Saber Hussain -- Immunocytochemistry, electron tomography, and energy dispersive x-ray spectroscopy (EDXS) on cryosections of human cancer cells doped with stimuli responsive polymeric nanogels loaded with iron oxide nanoparticles / Roberto Marotta [and others] -- Zwitterion siloxane to passivate silica against nonspecific protein adsorption / Zaki G. Estephan and Joseph B. Schlenoff -- Preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles / Xi-Jun Chen, Robert J. Hickey, and So-Jung Park -- Polyaspartic acid coated iron oxide nanoprobes for PET/MRI imaging / Taku Cowger and Jin Xie -- Ligand synthesis and passivation for silver and large gold nanoparticles for single-particle-based sensing and spectroscopy / Daniel Montiel [and others] -- Noncovalent intracellular drug delivery of hydrophobic drugs on au NPs / Tennyson Doane and Clemens Burda -- Modification of carbon nanotubes for gene delivery vectors / Victor Ramos-Perez [and others] -- Lipid-based nanoparticles as nonviral gene delivery vectors / Daniele Pezzoli [and others] -- Stabilizing gold nanoparticle bioconjugates in physiological conditions by PEGylation / Joan Comenge and Víctor F. Puntes
Summary The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology
Analysis chemie
chemistry
biotechnologie
biotechnology
nanotechnologie
nanotechnology
Chemistry (General)
Chemie (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Nanobiotechnology -- Laboratory manuals
Nanostructured materials -- Laboratory manuals
Manufactures.
Manufactured Materials
Phenomena and Processes
Publication Formats
Publication Characteristics
Technology, Industry, and Agriculture
Technology, Industry, Agriculture
Biological Phenomena
Nanostructures
Laboratory Manuals
Manufactures
Nanobiotechnology
Nanostructured materials
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hamad-Schifferli, Kimberly, editor.
Bergese, Paolo, 1975- editor.
ISBN 9781627034623
1627034625