| Description |
1 online resource (191 p.) |
| Contents |
Intro -- Table of Contents -- Abbreviations -- Abstract -- Kurzzusammenfassung -- 1 Introduction -- 1.1 Publications -- 2 Motion at the Nanoscale -- 2.1 Motion at Low Reynolds Numbers -- 2.2 Brownian Motion -- 3 Active Brownian motion -- 3.1 Self-Phoresis of Micro- and Nanoparticles -- 3.2 Active Diffusion Hypothesis for Enzymes -- 3.3 Molecular Catalysts & Tracer Diffusion -- 4 Diffusion Measurement Techniques -- 4.1 Fluorescence Correlation Spectroscopy -- 4.1.1 Single Molecule Fluorescence -- 4.1.2 Fluorescence Detection from a Confocal volume -- 4.1.3 Autocorrelation Function and Fitting |
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4.2 Nuclear Magnetic Resonance Spectroscopy -- 4.2.1 Nuclear Magnetization -- 4.2.2 Pulsed Field Gradients -- 4.2.3 Advanced Pulse Sequences -- 4.3 Other Techniques -- 4.3.1 Other Correlation Methods -- 4.3.2 Tracking Microscopy -- 4.3.3 Trapping Methods -- 4.3.4 Mutual Diffusion Measurement Techniques -- 4.3.5 Computational Methods -- 5 Enzymes Properties -- 5.1 Alkaline Phosphatase -- 5.2 Fructose-Bisphosphate Aldolase -- 5.3 Urease -- 5.4 FoF1-ATP synthase -- 6 Applications of Enzymes -- 6.1 Biocatalysis -- 6.2 Immobilization -- 6.3 Collective Effects -- 7 Misinterpretation of Enzyme FCS |
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7.1 Conspectus -- 7.2 Introduction -- 7.3 Experiment and Theory of the Enhanced Diffusion of ActiveEnzymes -- 7.4 Fluorescence Correlation Spectroscopy -- 7.5 Enhanced Diffusion of F1-ATPase -- 7.6 Effects and Artefacts in FCS Measurements of ProteinDiffusion -- 7.7 Suggestions for Future FCS Experiments of Enzyme Diffusion -- 7.8 Fluorescence Quenching of Alkaline Phosphatase -- 7.9 Conclusions -- 7.10 Acknowledgment -- 8 Label-Free Enzyme Diffusion Measurements -- 8.1 Abstract -- 8.2 Introduction -- 8.2.1 PFG-NMR Principle -- 8.3 Experimental Details -- 8.3.1 Materials |
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8.3.2 Enzyme Activity Assay -- 8.3.3 NMR and PFG-NMR Experiments -- 8.4 Results -- 8.4.1 Diffusion Measurements of Solutions in the Presence of Active Aldolase -- 8.4.2 Diffusion of the Enzyme Aldolase during Interaction with its InhibitorPyrophosphate -- 8.4.3 Diffusion of the Enzyme Aldolase during Substrate Conversion -- 8.5 Discussion -- 8.6 Conclusion -- 8.7 Acknowledgments -- 9 Enzyme Immobilization on Viruses -- 9.1 Abstract -- 9.2 Introduction -- 9.3 Results and Discussion -- 9.4 Conclusions -- 9.5 Materials and Methods -- 9.6 Acknowledgements -- 10 Misinterpretation of Molecular NMR |
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11 Conclusions -- 11.1 Active Diffusion of Enzymes and Chemical Catalysts -- 11.2 Enzyme-Phage-Colloids -- 11.3 Outlook -- A Appendix -- A.1 Enzyme Diffusion NMR Supplementary Information -- A.1.1 Aldolase Purification -- A.1.2 Aldolase Activity Assay -- A.1.3 Detailed Settings for the PFG-NMR Experiments -- A.1.4 Detailed Results of the PFG-NMR Experiments -- A.2 Enzyme-Phage-Colloid Supporting Information -- A.2.1. Genetic Modification of M13 Bacteriophages Templates -- A.2.2 Scanning and Transmission Electron Micrographs of Phage-Colloid |
| Notes |
Description based upon print version of record |
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A.2.3 Determination of Enzyme Loading on Phage-Colloids |
| Bibliography |
Includes bibliographical references |
| Subject |
Enzymes.
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Aldolase.
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Nanotechnology.
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Alkaline phosphatase.
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Enzymes
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Alkaline Phosphatase
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Nanotechnology
|
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enzyme.
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Aldolase
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Alkaline phosphatase
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Enzymes
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Nanotechnology
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| Form |
Electronic book
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| ISBN |
9783736963641 |
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3736963645 |
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