| Description |
1 online resource (x, 365 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1467 |
|
Springer protocols |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1467. 1064-3745
|
|
Springer protocols (Series)
|
| Contents |
WT1 mutation in childhood cancer / Jocelyn Charlton and Kathy Pritchard-Jones -- Clinical aspects of WT1 and the kidney / Eve Miller-Hodges -- Role of WT1 in embryonic development and normal organ homeostasis / Bettina Wilm and Ramon Muñoz-Chapuli -- Tools and techniques for Wt1-based lineage tracing / Bettina Wilm and Ramon Muñoz-Chapuli -- Biological systems and methods for studying WT1 in the epicardium / Víctor Velecela, Janat Fazal-Salom, and Ofelia M. Martínez-Estrada -- Isolation and colony formation of murine bone and bone marrow cells / Sophie McHaffie and You-Ying Chau -- Isolation and fluorescence-activated cell sorting of murine WT1-expressing adipocyte precursor cells / Louise Cleal and You-Ying Chau -- In vivo assays for assessing the role of the wilms' tumor suppressor 1 (Wt1) in angiogenesis / Richard J. McGregor [and others] -- Multiphoton microscopy for visualizing lipids in tissue / Martin Lee and Alan Serrels -- Function and regulation of the wilms tumor suppressor 1 (WT1) gene in fish / Birgit Perner [and others] -- Immunofluorescence staining of WT1 on sections of zebrafish embryos and larvae / Birgit Perner and Christoph Englert -- Fluorescence-activated cell sorting (FACS) protocol for podocyte isolation in adult zebrafish / Thomas J.D. Bates, Uta Naumann, and Christoph Englert -- In vitro transcription to study WT1 function / Stefan G.E. Roberts -- Measuring equilibrium binding constants for the WT1-DNA interaction using a filter binding assay / Paul J. Romaniuk -- Identifying direct downstream targets : WT1 ChIP-Seq analysis / Fabio da Silva, Filippo Massa, and Andreas Schedl -- WT1 associated protein-protein interaction networks / Ruthrothaselvi Bharathavikru and Alex von Kriegsheim -- Methods to identify and validate WT1-RNA interaction / Ruthrothaselvi Bharathavikru and Tatiana Dudnakova -- Bioinformatic analysis of next-generation sequencing data to identify WT1-associated differential gene and isoform expression / Stuart Aitken and Ruthrothaselvi Bharathavikru -- Immunotherapy targeting WT1 : designing a protocol for WT1 peptide-based cancer vaccine / Sumiyuki Nishida and Haruo Sugiyama |
| Summary |
This volume provides a thorough overview of the Wilms Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools. The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, The Wilms Tumour (WT1) Gene: Methods and Protocols is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed July 15, 2016) |
| Subject |
Nephroblastoma -- Genetic aspects -- Laboratory manuals
|
|
Wilms Tumor -- genetics
|
|
Genes, Wilms Tumor
|
|
WT1 Proteins
|
|
Medical genetics.
|
|
Oncology.
|
|
Medical -- Genetics.
|
|
Medical -- Oncology.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hastie, Nicholas, editor.
|
| ISBN |
9781493940233 |
|
1493940236 |
|
149394021X |
|
9781493940219 |
|
