| Description |
1 online resource (xv, 496 pages) : illustrations |
| Series |
Methods in molecular medicine ; 119 |
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Methods in molecular medicine ; 119.
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| Contents |
Identification of new papillomavirus types / Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst -- Identification of HPV variants / John Cason, Jon Bible, and Christine Mant -- Histochemical analysis of cutaneous HPV-associated lesions / Kiyofumi Egawa -- Histochemical analysis of cervical intraepithelial neoplasia / Michael Babawale [and others] -- Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections / Woei Ling Peh and John Doorbar -- Detection and quantitation of HPV gene expression using real-time PCR / Rashmi Seth [and others] -- Analysis of P16INK4A and integrated genomes as progression markers / Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz -- Use of biomarkers in the evaluation of CIN grade and progression of early CIN / Jan P.A. Baak and Arnold-Jan Kruse -- HPV DNA detection and typing in cervical scrapes / Peter J.F. Snijders [and others] -- HPV DNA detection and typing in inapparent cutaneous infections and premalignant lesions / Maurits de Koning [and others] -- Establishing HPV-containing keratinocyte cell lines from tissue biopsies / Margaret Anne Stanley -- Using an immortalized cell line to study the HPV lifecycle in organotypic "raft" cultures / Paul F. Lambert [and others] |
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Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose / Regina Wilson and Laimonis A. Laimins -- Propagation of infectious, high-risk HPV in organotypic "raft" culture / Margaret E. McLaughlin-Drubin and Craig Meyers -- Retrovirus-mediated gene transfer to analyze HPV gene regulation and protein functions in organotypic "raft" cultures / N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker -- The HPV xenograft severe combined immunodeficiency (scid) mouse model / William Bonner -- The cottontail rabbit papillomavirus model of high-risk HPV-induced disease / Janet L. Brandsma -- Studying the HPV lifecycle in 3A trophoblasts and resulting pathophysiology / Yong Liu, Hong You, and Paul L. Hermonat -- Replication and encapsidation of papillomaviruses in saccharomyces cerevisiae / Peter C. Angeletti -- Analysis of the regulation of viral transcription / Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard -- Analysis of HPV transcripts by RPA / Jason M. Bodily and Craig Meyers -- Analysis of regulatory motifs within HPV transcripts / Sarah A. Cumming and Sheila V. Graham -- Detection of HPV transcripts by nested RT-PCR / Christine Mant, Barbara Kell, and John Cason |
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Analysis of HPV DNA replication using transient transfection and cell-free assays / Bing Yuan Lin, Thomas R. Broker, and Louise T. Chow -- Detection and quantitation of HPV DNA replication by southern blotting and real-time PCR / Iain M. Morgan and Ewan R. Taylor -- Analysis of E7/RB associations / Sandra Caldeira, Wen Dong, and Massimo Tommasino -- Transformation assays for HPV oncoproteins / Paola Massimi and Lawrence Banks -- Analysis of adeno-associated virus and hpv interaction / Paul L. Hermonat [and others] -- In vitro assays of substrate degradation induced by high-risk HPV E6 oncoproteins / Miranda Thomas and Lawrence Banks -- Measuring the induction or inhibition of apoptosis by HPV proteins / Anna M. Kowalczyk [and others] -- Codon optimization of papillomavirus genes / Martin Muller -- Generation of HPV pseudovirions using transfection and their use in neutralization assays / Christopher B. Buck [and others] -- Generation and application of HPV pseudovirions using vaccinia virus / Martin Sapp and Hans-Christoph Selinka |
| Summary |
Human papillomaviruses (HPV) are an important class of pathogens responsible for a variety of diseases, including cervical cancer, the second most commonly found female cancer worldwide. In Human Papillomaviruses: Methods and Protocols, leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of this formidable virus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Human Papillomaviruses: Methods and Protocols offers both novice and experienced investigators a set of highly successful analytical tools for unlocking the secrets of the human papillomaviruses and their pathologies |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Papillomavirus diseases -- Laboratory manuals
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Papillomaviruses -- Laboratory manuals
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Alphapapillomavirus -- genetics
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Alphapapillomavirus -- pathogenicity
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Uterine Cervical Neoplasms -- virology
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MEDICAL -- Infectious Diseases.
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HEALTH & FITNESS -- Diseases -- Contagious.
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Papillomavirus diseases
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Papillomaviruses
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Davy, Clare
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Doorbar, John
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| LC no. |
2005006265 |
| ISBN |
1588293734 |
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9781588293732 |
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9781592599820 |
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1592599826 |
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