Title Antibacterial peptide protocols / edited by William M. Shafer

Published Totowa, N.J. : Humana Press, ©1997

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Description 1 online resource (x, 259 pages) : illustrations Series Methods in molecular biology ; v. 78 Methods in molecular biology (Clifton, N.J.) ; v. 78 Contents Origins and development of peptide antibiotic research : from extracts to abstracts to contracts / John K. Spitznagel -- HPLC methods for purification of antimicrobial peptides / Michael E. Selsted -- Strategies for the isolation and characterization of antimicrobial peptides of invertebrates / Charles Hetru, Philippe Bulet -- Big defensin and tachylectins-1 and -2 / Shun-ichiro Kawabata, Sadaaki Iwanaga -- Tachyplesin and anti-lipopolysaccharide factor / Tatsushi Muta, Sadaaki Iwanaga -- Circular dichroism studies of secondary structure of peptides / Martha M. Juban, Maryam M. Javadpour, Mary D. Barkley -- Analytical ultracentrifugation studies of association of peptides / Martha M. Juban, Maryam M. Javadpour, Mary D. Barkley -- NMR characterization of amphipathic helical peptides / Xiaotang Wang, Kathleen M. Morden -- Laboratory production of antimicrobial peptides in native conformation / Erika V. Valore, Tomas Ganz -- An approach combining rapid cDNA amplification and chemical synthesis or the identification of novel, cathelicidin-derived, antimicrobial peptides / Alessandro Tossi, Marco Scocchi, Margherita Zanetti, Renato Gennaro, Paola Storici, Domenico Romeo -- Molecular biological strategies in the analysis of antibiotic peptide gene families : the use oligonucleotides as hybridization probes / Charles L. Bevins, Gill Diamond -- Designer assays for antimicrobial peptides: disputing the "one-size-fits-all" theory / Deborah A. Steinberg, Robert I. Lehrer -- Interaction of cationic peptides with bacterial membranes / Shafique Fidai, Susan W. Farmer, Robert E. Hancock -- The genetic basis of microbial resistance to antimicrobial peptides / Eduardo A. Groisman, Arden Aspedon -- Assay of antibacterial activities of the bactericidal/permeability-increasing protein in natural biological fluids / Jerrold Weiss -- Assay systems for measurement of chemotactic activity / H. Anne Pereira -- Neutralization of the in vivo activity of E. coli-derived lipopolysaccharide by cationic peptides / Daniel J. Brackett, Megan R. Lerner, H. Anne Pereira Summary In Antibacterial Peptide Protocols, leading authorities review for the first time in one volume all the major biochemical, molecular, bacteriological, and physical techniques available to assess antimicrobial peptides. These state-of-the-art methods ensure easily reproducible results in such important procedures as the isolation and characterization of antimicrobial peptides, the molecular characterization of genes encoding antimicrobial peptides, and the use of expression systems to isolate peptides. Bioassays and microbial genetic techniques are also included, as are antibacterial assays as the final readout system. These methods detailed in Antibacterial Peptide Protocols will play an important role in the treatment of infectious diseases, particularly with the increasing problem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies Analysis moleculaire biologie molecular biology micro-organismen microorganisms bacteria classificatie classification taxonomie taxonomy Proteins and Enzymes Eiwitten en enzymen Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Peptide antibiotics -- Research -- Methodology Peptide antibiotics -- Analysis Genetics -- Technique. Anti-Bacterial Agents -- analysis Peptides -- analysis Genetic Techniques Microbiological Techniques Genetics -- Technique Peptide antibiotics -- Analysis Peptiden. Antibacteriële middelen. Infectieziekten. Wetenschappelijke technieken. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Shafer, William M. ISBN 9781592595648 1592595642 1280836830 9781280836831 9786610836833 6610836833

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