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E-book

Title Basic protein and peptide protocols / edited by John M. Walker
Published Totowa, N.J. : Humana Press, ©1994

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Description 1 online resource (xii, 490 pages) : illustrations
Series Methods in molecular biology ; v. 32
Methods in molecular biology (Clifton, N.J.) ; v. 32
Contents Lowry method for protein quantitation -- Bicinchoninic acid (BCA) assay for protein quantitation -- Bradford method for protein quantitation -- Nondenaturing polyacrylamide gel electrophoresis of proteins -- SDS polyacrylamide gel electrophoresis of proteins -- Gradient SDS polyacrylamide gel electrophoresis of proteins -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins -- Electrophoresis of peptides -- Isoelectric focusing of proteins in ultra-thin polyacrylamide gels -- In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis -- Two-dimensional polyacrylamide gel electrophoresis of proteins -- Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension -- Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension -- Quantification of proteins on polyacrylamide gels (nonradioactive) -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique -- identification of glycoproteins on nitrocellulose membranes and gels -- Release of oligosaccharides from glycoproteins -- Structural profiling of oligosaccharides of glycoproteins -- Dying gels -- Fluorography of polyacrylamide gels containing tritium -- Electrophoretic elution of proteins from polyacrylamide gels -- Preparation of proteins from gels for protein microsequencing -- Comparison of protein primary structures: peptide mapping -- Protein blotting -- Detection of polypeptides on immunoblots using secondary antibodies or protein A -- Detection of proteins on protein blots using chemiluminescent systems -- Detection of proteins on blots using gold or immunogold -- Amino acid analysis -- Molecular-weight estimation for native proteins using size-exclusion high-performance liquid chromatography -- Peptide purification by reverse-phase HPLC -- Enzymatic methods for cleaving proteins -- Chemical cleavage of proteins -- Chemical modification of proteins -- Dansyl method for identifying N-terminal amino acids -- Dansyl-Edman method for peptide sequencing -- C-terminal sequencing of peptides: the thiocyanate degradation method -- Analysis of cysteine residues and disulfide bonds -- Preparation and characterization of monoclonal antibodies to proteins and other cellular components -- Raising of a polyclonal antiserum to a protein -- Production of antisera to synthetic peptides -- Production of antibodies using proteins in gel bands -- Preparation of purified immunoglobulin G (IgG) -- Enzyme and biotin labeling of antibody -- Labeling of peptides and proteins by radioiodination -- Radioimmunoassay of peptides and proteins -- Enzyme-linked immunosorbent assay (ELISA) -- Free zone capillary electrophoresis
Summary Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Analysis eiwitten
proteins
peptiden
peptides
analytische methoden
analytical methods
aminozuursequenties
amino acid sequences
Proteins and Enzymes
Eiwitten en enzymen
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
English
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Proteins -- Analysis -- Methodology
Peptides -- Analysis -- Methodology
Proteins -- analysis
Peptides -- analysis
Proteins -- Analysis -- Methodology.
Eiwitten.
Peptiden.
Analyse (algemeen)
Wetenschappelijke technieken.
Animal Biochemistry.
Human Anatomy & Physiology.
Health & Biological Sciences.
Form Electronic book
Author Walker, John M., 1948-
LC no. 93048929
ISBN 9781592595198
1592595197
0896032698
9780896032699