| Description |
1 online resource (xxxvii, 850 pages) : illustrations |
| Series |
Methods in enzymology, 0076-6879 ; v. 159 |
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Methods in enzymology ; v. 159. 0076-6879
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| Contents |
t Direct cytochemical localization of the free catalytic subunit of cAMP-dependent protein kinase / r Craig V. Byus ... [et al.] -- t Direct cytochemical localization of regulatory subunit of cAMP-dependent protein kinase using fluoresceinated catalytic subunit / r William H. Fletcher ... [et al.] -- t Identification and ligand-induced modification of the cAMP receptor in Dictyostelium / r Peter Klein ... [et al.] -- t Crystallizing catabolite gene activator protein with cAMP for structural analysis / r Irene T. Weber, Jackie D. Corbin and Roger A. Johnson -- t Isolation of a Trypanosoma cAMP-binding protein which is not a regulatory subunit of cAMP-dependent protein kinase / r Rafael Rangel-Aldao ... [et al.] -- t Application of molecular genetic techniques to the cAMP-dependent protein kinase system / r G. Stanley McKnight ... [et al.] -- t Cloning of cDNA for the catalytic subunit of cAMP-dependent protein kinase / r Mark O. Showers ... [et al.] -- t Molecular cloning of cDNA for a hormone-regulated isoform of the regulatory subunit of type II cAMP-dependent protein kinase from rat ovaries / r Tore Jahnsen ... [et al.] -- t Expression and mutagenesis of the regulatory subunit of cAMP-dependent protein kinase in Escherichia coli / r Lakshmi D. Saraswat ... [et al.] -- t Methods to distinguish various types of protein phosphatase activity / r David L. Brautigan ... [et al.] -- t Preparation of protein phosphatase-resistant substrates using adenosine 5'-O-([gamma]-Thio)triphosphate / r Heng-Chun Li ... [et al.] -- t Preparation of low-molecular -weight forms of rabbit muscle protein phosphatase / r Anthony DeGuzman ... [et al.] -- t Purification of glycogen-bound high-molecular-weight phosphoprotein phosphatase from rabbit skeletal muscle / r Balwant S. Khatra, Jackie D. Corbin and Roger A. Johnson -- t Purification and characterization of phosphorylase phosphatase from rabbit skeletal muscle / r Steven J. McNall ... [et al.] |
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t Liver Low-Km, hormone-sensitive phosphodiesterase / r E.G. Loten, Jackie D. Corbin and Roger A. Johnson -- t Insulin-sensitive CAMP phosphodiesterase in rat adipose tissue / r Tetsuro Kono, Jackie D. Corbin and Roger A. Johnson -- t Isolation and characterization of insulin-stimulated, high-affinity cAMP phosphodiesterases from rat liver / r Miles D. Houslay ... [et al.] -- t Preparation of dog kidney high-affinity cAMP phosphodiesterase / r W.J. Thompson ... [et al.] -- t Brain low-Km cAMP phosphodiesterase / r Philippe Demazancourt ... [et al.] -- t Purification of cAMP phosphodiesterase from platelets / r Paul G. Grant ... [et al.] -- t Zinc-containing cyclic nucleotide phosphodiesterases from bakers' yeast / r John Londesborough ... [et al.] -- t mutational analysis of phosphodiesterase in Drosophila / r Ronald L. Davis, Jackie D. Corbin and Roger A. Johnson |
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t Protein phosphatase-1 and protein phosphatase-2A from rabbit skeletal muscle / r Philip Cohen ... [et al.] -- t Protein phosphatase-2B from rabbit skeletal muscle: A Ca2+-Dependent, Calmodulin-Stimulated Enzyme / r Alexander A. Stewart ... [et al.] -- t Protein phosphatase-2C from rabbit skeletal muscle and liver: An Mg2+-dependent enzyme / r Clare H. McGowan ... [et al.] -- t Protein phosphatase inhibitor-1 and inhibitor-2 from rabbit skeletal muscle / r Philip Cohen ... [et al.] -- t Purification of an Mg2+-dependent protein phosphatase / r Shigeru Tsuiki ... [et al.] -- t Purification of smooth muscle myosin phosphatase from Turkey gizzard / r Mary D. Pato ... [et al.] -- t Assay of cyclic nucleotide phosphodiesterase using radiolabeled and fluorescent substrates / r Randall L. Kincaid ... [et al.] -- t Analysis of phosphodiesterase reaction mixtures by high-performance liquid chromatography / r D. Martin Watterson ... [et al.] -- t Cyclic nucleotide phosphodiesterase activity: Histochemical and cytochemical methods / r Satoki Ueno ... [et al.] -- t Methylxanthine inhibitors of phosphodiesterases / r Jack N. Wells ... [et al.] -- t Acylpeptide inhibitors of phosphodiesterase produced by Bacillus subtilis / r Kuniaki Hosono, Jackie D. Corbin and Roger A. Johnson -- t Selective inhibitors of specific phosphodiesterases in intact adipocytes / r Vincent Manganiello ... [et al.] -- t Cyclic nucleotide analogs used to study phosphodiesterase catalytic and allosteric sites / r Christophe Erneux ... [et al.] -- t cAMP analogs used to study low-Km, hormone-sensitive phosphodiesterase / r Stephen J. Beebe ... [et al.] |
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t Purification of calmodulin-stimulated cyclic nucleotide phosphodiesterase by monoclonal antibody affinity chromatography / r R. Scott Hansen ... [et al.] -- t Purification and properties of calmodulin-activated cyclic nucleotide phosphodiesterase from mammalian brain / r Randall L. Kincaid ... [et al.] -- t Purification of calmodulin-stimulated phosphodiesterase by affinity chromatography on calmodulin fragment 1-77 linked to sepharose / r Giulio Draetta ... [et al.] -- t Isolation of bovine brain calmodulin-dependent cyclic nucleotide phosphodiesterase isozymes / r Rajendra K. Sharma ... [et al.] -- t Estimating the association of phosphodiesterase with calmodulin in intact cells / r Jerry R. Miller ... [et al.] -- t Preparation of fluorescent, cross-linking, and biotinylated calmodulin derivatives and their use in studies of calmodulin-activated phosphodiesterase and protein phosphatase / r Randall L. Kincaid ... [et al.] -- t Preparation, characterization, and properties of affinity-purified antibodies to calmodulin-dependent cyclic nucleotide phosphodiesterase and the protein phosphatase calcineurin / r Randall L. Kincaid, Jackie D. Corbin and Roger A. Johnson -- t Selective inhibitors of calmodulin-dependent phosphodiesterase and other enzymes / r Hiroyoshi Hidaka ... [et al.] -- t Heat-sensitive inhibitor of calmodulin-regulated cyclic nucleotide phosphodiesterase / r Eugenia Jedlicki ... [et al.] -- t Preparation and use of iodinated calmodulin for studies of calmodulin-binding proteins / r LindaJ Van Eldik, Jackie D. Corbin and Roger A. Johnson -- t High-affinity, calmodulin-dependent isoforms of cyclic nucleotide phosphodiesterase in rat testis / r Kenneth Purvis ... [et al.] -- t Isolation and comparison of bovine heart cGMP-inhibited and cGMP-stimulated phosphodiesterases / r S.A. Harrison ... [et al.] -- t Purification of rod outer segment GTP-Binding protein subunits and cgmp phosphodiesterase by single-step column chromatography / r Akio Yamazaki ... [et al.] -- t Platelet cGMP-binding phosphodiesterase / r Pavel Hamet ... [et al.] -- t Purification of cGMP-binding protein phosphodiesterase from rat lung / r Sharron H. Francis ... [et al.] -- t Photoaffinity labeling of high-affinity cGMP-specific noncatalytic binding sites on cGMP phosphodiesterase of Rod Outer segments / r Akio Yamazaki ... [et al.] |
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t Role of cAMP in cyclic cascade regulation / r Emily Shacter ... [et al.] -- t Theoretical considerations of the regulatory steps in the cAMP cascade system / r Steṕhane Swillens ... [et al.] -- t Yeast mutants altered in the cAMP cascade system / r Tatsuo Ishikawa ... [et al.] -- t Improvements in the automated radioimmunoassay for CAMP or cGMP / r Gary Brooker, Jackie D. Corbin and Roger A. Johnson -- t cAMP turnover in intact cells / r R. Barber ... [et al.] -- t Monitoring cyclic nucleotide metabolism in intact cells by 18O labeling / r Timothy F. Walseth ... [et al.] -- t Purification and assay of cAMP, cGMP, and cyclic nucleotide analogs in cells treated with cyclic nucleotide analogs / r Jackie D. Corbin ... [et al.] -- t cAMP export and its regulation by prostaglandin A1 / r Laurence L. Brunton ... [et al.] -- t Use of antibody- sepharose columns to study hormonal activation of cAMP-dependent protein kinase isozymes / r Roald Ekanger ... [et al.] -- t Selective activation of the cAMP-dependent protein kinase isoenzymes / r S.A. Livesey ... [et al.] -- t Use of synergistic pairs of site-selective cAMP analogs in intact cells / r Stephen J. Beebe ... [et al.] -- t cAMP-dependent protein kinase regulatory subunit phosphorylation in intact cells / r Marc C. Mumby ... [et al.] -- t Ammonium sulfate precipitation assay for the study of cyclic nucleotide binding to proteins / r Stein Ove Døskeland ... [et al.] -- t cGMP-dependent protein kinase activation in intact tissues / r Ronald R. Fiscus ... [et al.] -- t cAMP analog antagonists of cAMP action / r Lynne H. Parker Botelho ... [et al.] -- t Peptide inhibitors of CAMP-dependent protein kinase / r Bruce E. Kemp ... [et al.] -- t Type II cAMP-dependent protein kinase regulatory subunit-binding proteins / r Suzanne M. Lohmann ... [et al.] -- t Use of digitonin-permeabilized adipocytes for cAMP studies / r Robert A. Mooney, Jackie D. Corbin and Roger A. Johnson -- t Purification of cAMP-free and cAMP-bound forms of bovine heart cAMP-dependent protein kinase holoenzyme / r Charles E. Cobb ... [et al.] -- t Preparation of regulatory subunits from bovine heart cAMP-dependent protein kinase by a nondenaturing method / r Mark Seville ... [et al.] -- t Purification of Drosophila cAMP-dependent protein kinase / r John L. Foster ... [et al.] -- t Using immunocolloidal gold electron microscopy to investigate cAMP-dependent protein kinase cellular compartmentalization / r Richard A. Jungmann ... [et al.] |
| Summary |
Novel approaches for the study of phosphodiesterases and phosphoprotein phosphatases, as well as those of nonmammalian cyclic nucleotide receptors, are emphasized for the first time in the Methods of Enzymology series. New methods for the study of protein kinases are also presented |
| Analysis |
Acido nucleico |
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DNA |
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Nucelotodeo |
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Nucleic acid |
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Nucleotide |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Cyclic nucleotides.
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Phosphatases.
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Nucleotides, Cyclic -- isolation & purification.
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Nucleotides, Cyclic -- metabolism.
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Phosphoric Diester Hydrolases -- isolation & purification.
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| Form |
Electronic book
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| Author |
Corbin, Jackie D.
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Johnson, Roger A
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| ISBN |
0121820602 (electronic bk.) |
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9780121820602 (electronic bk.) |
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