Limit search to available items
Book Cover
E-book

Title Protein phosphorylation : a practical approach / edited by D.G. Hardie
Edition 2nd ed
Published London ; New York : Oxford University Press, 1999

Copies

Description 1 online resource (xxviii, 431 pages) : illustrations
Series The practical approach series ; 211
Practical approach series ; 211.
Contents Study of protein phosphorylation in intact cells / Timothy A.J. Haystead -- Analysis of signal transduction pathways using protein kinase inhibitors and activators / Yasuhito Naito -- Analysis of signal transduction pathways using protein-serine/threonine phosphatase inhibitors / D. Grahame Hardie -- Analysis of signal transduction pathways using molecular biological manipulations / Calum Sutherland -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates / Peter van der Geer -- Phosphorylation site analysis by mass spectrometry / Ken I. Mitchelhill -- Assay and purification of protein (serine/threonine) phosphatases / Carol Mackintosh -- Identification of substrates for protein-tyrosine phosphates / Andrew J. Garton -- Assay and purification of protein-serine/threonine kinases / D. Grahame Hardie -- Assay of protein kinases and phosphatases using specific peptide substrates / Maria Ruzzene -- Cloning and expression of cDNAs encoding protein kinase subunits / David Carling -- Interaction cloning of protein kinase partners / Julie M. Stone -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation / Rikiro Fukunaga -- Analysis of protein kinase interactions using the two-hybrid method / Rong-Jiang -- Analysis of protein kinase interactions using biomolecular interaction analysis / Friedrich -- Analysis of protein kinase specificity using oriented peptide libraries / Zhou Songyang -- Crystallization of protein kinases and phosphatases / Bostijan Kobe
Summary Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Phosphoproteins -- Laboratory manuals
Phosphorylation -- Laboratory manuals
Protein kinases -- Laboratory manuals
Phosphoprotein phosphatases -- Laboratory manuals
Phosphorylation.
Protein kinases.
Phosphorylation
Protein Kinases
SCIENCE -- Life Sciences -- Biochemistry.
Phosphoprotein phosphatases
Phosphoproteins
Phosphorylation
Protein kinases
Methode
Phosphorylierung
Proteine
Proteinkinasen
Fosforylering.
Eiwitten.
Proteïne-fosfatasen.
Eiwitkinasen.
Protéines.
Phosphoprotéine phosphatase.
Protéine kinase.
Phosphorylation.
Phosphoprotéines -- Manuels de laboratoire.
Protéines kinase -- manuels de laboratoire.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hardie, D. G. (D. Grahame)
LC no. 99032319
ISBN 0585483930
9780585483931
0191565903
9780191565908
9786610375677
6610375674
1280375671
9781280375675