Title Telomerase inhibition : strategies and protocols / edited by Lucy G. Andrews and Trygve O. Tollefsbol

Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (x, 193 pages) : illustrations Series Methods in molecular biology ; v. 405 Methods in molecular biology (Clifton, N.J.) ; v. 405. 1064-3745 Contents Methods of telomerase inhibition -- Telomerase inhibition by synthetic nucleic acids and chemosensitization in human bladder cancer cell lines -- hTERT knockdown in human embryonic kidney cells using double-stranded RNA -- RNA interference using a plasmid construct expressing a short-hairpin RNA -- Retrovirus-mediated RNA interference -- Telomerase inhibition and telomere targeting in hematopoietic cancer cell lines with small non-nucleosidic synthetic compounds (BIBR1532) -- Uses of telomerase peptides in anti-tumor immune therapy -- Establishing cell-based reporter systems for the analysis of hTERT expression -- Telomerase RNA inhibition using antisense oligonucleotide against human telomerase RNA linked to a 2',5'-oligoadenylate -- Knockdown of telomerase RNA using hammerhead ribozymes and RNA interference -- Evaluation of tankyrase inhibition in whole cells -- Inhibition of telomerase by targeting MAP kinase signaling -- Screening of telomerase inhibitors -- Telomerase inhibition combined with other chemotherapeutic reagents to enhance anti-cancer effect Summary Due in part to the selective nature of telomerase inhibition as an anticancer approach, the field has expanded considerably in the past decade. The recent advances in methods of telomerase inhibition encompass many different areas of research including molecular biology, cell biology, biochemistry, oncology and gerontology. Telomerase Inhibition provides methods and protocols for those researchers. The techniques described in this book should provide the researcher with a diverse and comprehensive set of tools with which to study telomerase inhibition. Leaders in the field provide recently developed methods that have widespread application such as targeting the telomerase holoenzyme, its RNA template and other elements associated with telomerase activity. Additional methods involving the screening of telomerase inhibitors and telomerase inhibition combined with other chemotherapeutic agents are presented. This text, on the cutting edge of the field, will provide investigators with the most recent methods applied to the expanding field of telomerase inhibition Analysis telomeren telomeres enzymen enzymes reverse transcriptase remmers reverse transcriptase inhibitors enzymremmers enzyme inhibitors remming inhibition reverse transcriptase biologische technieken biological techniques protocollen protocols Molecular Genetics Moleculaire genetica Bibliography Includes bibliographical references and index Notes English Print version record Subject Telomerase -- Inhibitors Telomerase -- Laboratory manuals Telomerase. Oligonucleotides. Chemotherapy. Nucleic acids. Bladder -- Diseases. Nucleoproteins. Therapeutics. Reverse transcriptase. Nucleotides. Urinary organs -- Diseases. Genitourinary organs -- Diseases. Carrier proteins. Proteins. Diseases. Phosphotransferases. Transferases. Enzymes. Urinary Bladder Neoplasms Telomerase Oligonucleotides Drug Therapy Polynucleotides Urologic Neoplasms Urinary Bladder Diseases Ribonucleoproteins Therapeutics RNA-Directed DNA Polymerase RNA-Binding Proteins Urogenital Neoplasms Nucleotides Urologic Diseases Analytical, Diagnostic and Therapeutic Techniques and Equipment DNA-Directed DNA Polymerase Nucleic Acids, Nucleotides, and Nucleosides Female Urogenital Diseases Male Urogenital Diseases Carrier Proteins Nucleoproteins DNA Nucleotidyltransferases Neoplasms by Site Proteins Chemicals and Drugs Disease Nucleotidyltransferases Female Urogenital Diseases and Pregnancy Complications Neoplasms Phosphotransferases Amino Acids, Peptides, and Proteins Transferases Enzymes Enzymes and Coenzymes protein. enzyme. treating (health care function) Urinary organs -- Diseases Transferases Therapeutics Reverse transcriptase Proteins Phosphotransferases Oligonucleotides Nucleotides Nucleoproteins Nucleic acids Genitourinary organs -- Diseases Enzymes Diseases Chemotherapy Carrier proteins Bladder -- Diseases Telomerase Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Andrews, Lucy G. Tollefsbol, Trygve O. ISBN 9781603270700 1603270701 9781597455015 1597455016

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