Title Low-cost methods for molecular characterization of mutant plants : tissue desiccation, DNA extraction and mutation discovery: protocols / Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger

Published Cham : SpringerOpen, 2015

Online access available from: SpringerOpen books    View Resource Record

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Description 1 online resource (x, 35 pages) : illustrations (some color) Series Online access with purchase: Springer (t) Contents Introduction -- Health and Safety Considerations -- Sample Collection and Storage -- Low-Cost DNA Extraction -- PCR Amplification for Low-Cost Mutation Discovery -- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants -- Example Data -- Conclusions Summary This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits Bibliography Includes bibliographical references Notes Online resource; title from PDF title page (SpringerLink, viewed May 13, 2015) Subject Plant hybridization -- Molecular aspects. Plant molecular genetics. Form Electronic book Author Till, Bradley J., author ISBN 3319162586 3319162594 (electronic bk.) 9783319162584 9783319162591 (electronic bk.)

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