| Description |
1 online resource (xvi, 558 pages) : illustrations |
| Series |
Methods in molecular biology ; 171 |
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Methods in molecular biology (Clifton, N.J.) ; v. 171.
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| Contents |
Isolation of proteoglycans from cell cultures and tissues / M. Yanagishita -- Isolation of proteoglycans from tendon / K.G. Vogel and J.A. Peters -- Purification of proteoglycans from mineralized tissues / N.S. Fedarko -- Purification of perlecan from endothelial cells / J. Whitelock -- Isolation and characterization of nervous tissue proteoglycans / Y. Yamaguchi -- Analysis of proteoglycans and glycosaminoglycans from drosophila / W.D. Staatz [and others] -- Cartilage and smooth muscle cell proteoglycans detected by affinity blotting using biotinylated hyaluronan / J. Melrose -- Preparation of proteoglycans for N-terminal and internal amino acid sequence analysis / P.J. Neame -- High-specific-activity 35S-labeled heparan sulfate prepared from cultured cells / N.W. Shworak -- Selective detection of sulfotransferase isoforms by the ligand affinity-conversion approach / N.W. Shworak -- Particulate and soluble glycosaminoglycan-synthesizing enzymes. Preparation assay and use / G. Sugumaran and J.E. Silbert -- Disaccharide composition of hyaluronan and chondroitin/dermatan sulfate. Analysis with fluorophore-assisted carbohydrate electrophoresis / A.H. Plaas [and others] -- Integral glycan sequencing of heparan sulfate and heparin saccharides / J.E. Turnbull -- Analytical and preparative strong anion-exchange HPLC of heparan sulfate and heparin saccharides / J.E. Turnbull -- Proteoglycans analyzed by composite gel electrophoresis and immunoblotting / G.R. Dodge and R. Heimer -- Quantitation of proteoglycans in biological fluids using alcian blue / M. Karlsson and S. Bjornsson -- Cellulose acetate electrophoresis of glycosaminoglycans / Y. Wegrowski and F.X. Maquart -- Disaccharide composition in glycosaminoglycans/proteoglycans analyzed by capillary zone electrophoresis / N.K. Karamanos and A. Hjerpe -- Intact and oligomeric glycosaminoglycans analyzed with capillary electrophoresis / N.K. Karamanos and A. Hjerpe -- Recombinant expression of proteoglycans in mammalian cells. Utility and advantages of the vaccinia virus/T7 bacteriophage hybrid expression system / D.J. McQuillan [and others] -- Purification of recombinant human decorin and its subdomains / A.L. McBain and D.M. Mann -- Prokaryotic expression of proteoglycans / A.D. Murdoch and R.V. Iozzo -- Proteoglycan gene targeting in somatic cells / G. Ghiselli and R.V. Iozzo -- Constitutive and inducible antisense expression / M. Handler and R.V. Iozzo -- Cell-mediated transfer of proteoglycan genes / J.W. Fischer [and others] -- Morphological evaluation of proteoglycans in cells and tissues / S.L. Lara, S.P. Evanko and T.N. Wight -- Expression and characterization of engineered proteoglycans / W. Luo [and others] -- Intracellular localization of engineered proteoglycans / T.L. Chen [and others] -- Selection of glycosaminoglycan-deficient mutants / X. Bai, B. Crawford and J.D. Esko -- Xyloside priming of glycosaminoglycan biosynthesis and inhibition of proteoglycan assembly / T.A. Fritz and J.D. Esko -- Inhibition of heparan sulfate synthesis by chlorate / H.E. Conrad -- Detection of proteoglycan core proteins with glycosaminoglycan lyases and antibodies / J.R. Couchman and P. Tapanadechopone -- Proteoglycan core proteins and catabolic fragments present in tissues and fluids / J.D. Sandy -- Degradation of heparan sulfate by nitrous acid / H.E. Conrad -- Degradation of heparan sulfate with heparin lyases / L.A. LeBrun and R.J. Linhardt -- Degradation of chondroitin sulfate and dermatan sulfate with chondroitin lyases / M.J. Hernaiz and R.J. Linhardt -- In vitro assays for hyaluronan synthase / A.P. Spicer -- Hyaluronidase activity and hyaluronidase inhibitors. Assay using a microtiter-based system / S.S. Nawy [and others] -- Detecting hyaluronidase and hyaluronidase inhibitors. Hyaluronan-substrate gel and -inverse substrate gel techniques / K. Mio [and others] -- Affinity coelectrophoresis of proteoglycan-protein complexes / J.D. San Antonio and A.D. Lander -- Binding constant measurements for inhibitors of growth factor binding to heparan sulfate proteoglycans / K.E. Forsten and M.A. Nugent -- Interaction of proteoglycans with receptor tyrosine kinases / D.K. Moscatello and R.V. Iozzo -- Measuring single-cell cytosolic ca2+ concentration in response to proteoglycans / S. Patel and R.V. Iozzo -- Serum amyloid a peptide interactions with glycosaminoglycans. Evaluation by affinity chromatography / J.B. Ancsin and R. Kisilevsky -- Interactions of lipoproteins with proteoglycans / K.J. Williams -- Hyaluronan and hyaluronan-binding proteins. Probes for specific detection / B.P. Toole, Q. Yu and C.B. Underhill -- Novel confocal-FRAP analysis of carbohydrate-protein interactions within the extracellular matrix / P. Gribbon, B.C. Heng and T.E. Hardingham -- Regulatory roles of syndecans in cell adhesion and invasion / J.K. Langford and R.D. Sanderson -- Optical biosensor techniques to analyze protein-polysaccharide interactions / D.G. Fernig -- Phage display technology to obtain antiheparan sulfate antibodies / T.H. van Kuppevelt [and others] -- Tissue-specific binding by FGF and FGF receptors to endogenous heparan sulfates / A. Friedl, M. Filla and A.C. Rapraeger |
| Summary |
Proteoglycans, highly complex macromolecules found in mammalian and lower organisms, serve a multitude of biological functions. In Proteoglycan Protocols, Renato Iozzo, MD, and an authoritative team of investigators present for the first time a comprehensive and up-to-date collection of readily reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring step-by-step protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, and to generate strategies for prokaryotic and eukaryotic expression. There are also detailed techniques for the suppression of specific proteoglycan genes, for the detection of mutant cells and their degradation products, and for studying specific interactions between proteoglycans and extracellular matrix proteins and with growth factors and their receptors. Each method is carefully described by leading experts in the field and includes practical considerations that are often lacking in formal papers. Authoritative and detailed, Proteoglycan Protocols offers molecular and cellular biologists, biochemists, pathologists, and geneticists a comprehensive reference and practical guide to the most common methods used today for the isolation and characterization of proteoglycans from cultured cells, tissues, and biological fluids |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Proteoglycans -- Laboratory manuals
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Proteoglycans -- analysis
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Proteoglycans -- chemistry
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Proteoglycans -- isolation & purification
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SCIENCE -- Life Sciences -- Biochemistry.
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Proteoglycans
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Wetenschappelijke technieken.
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Iozzo, Renato V., 1950-
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| LC no. |
00061317 |
| ISBN |
9781592592098 |
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1592592090 |
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0585403198 |
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9780585403199 |
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9786610830190 |
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6610830193 |
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1280830190 |
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9781280830198 |
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