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Title RNA viruses : a practical approach / edited by Alan J. Cann
Published Oxford ; New York : Oxford University Press, 2000

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Description 1 online resource (xx, 266 pages) : illustrations
Series The practical approach series ; 226
Practical approach series ; 226.
Contents Intro; Contents; List of protocols; Abbreviations; 1 Investigation of RNA virus genome structure; 1 Introduction-the nature of the virus genome; Properties of the genomes of RNA viruses; Properties of the virions of RNA viruses; Paradigm for analysis of RNA virus genome structure; 2 Growth, assay, and purification of RNA viruses; Source of virus: in vivo versus in vitro methods; Assay of virus yield; Harvesting and concentrating of virus; Purification of virus; Radiolabelling; 3 RNA extraction; Genome RNA extraction from virions; Genome RNA extraction without virion purification
4 Fractionation of RNA, and analysis by Northern (RNA) blottingFractionation of RNA; Northern blotting and detection with labelled probes; 5 Further analysis; Characterization by restriction-endonuclease digest patterns; Characterization by ribonuclease protection; References; 2 Mutagenesis of RNA virus genomes; 1 Introduction; 2 Generation of RNA virus mutants; Selection of mutants from the RNA quasispecies; Recombination of RNA virus genomes; Reassortment of segmented RNA virus genomes; Generation of defective RNA virus populations by passage in culture
Recovery of RNA virus mutants from infectious cDNA3 Determining the genotype of RNA virus mutants; 4 Analysis of the frequency of RNA virus mutants; References; 3 Analysis of transcriptional control In RNA virus Infections; 1 Introduction; 2 Analysis of virus mRNAs in virus-infected cells; General considerations; Radiolabelling and extraction of virus RNAs; Gel electrophoresis of RNAs; 3 Analysis of virus mRNA structure; General considerations; Separation of virus RNA in preparative agarose gels; Extraction of virus RNA from gel slices; One-dimensional oligonucleotide fingerprinting
Northern blot hybridization4 The use of RNA reporter constructs for transcriptional assays; RNA transcription in vitro; RNA transfection of DI RNA construct and helper virus infection; DNA transfection and vaccinia virus infection; CAT reporter assay system; Detection and quantitation of minute amount of virus RNAs; References; 4 Analysis of RNA virus-encoded proteinases; 1 Introduction; 2 Defining the proteinase type; Inhibitor studies; Sequence analysis; 3 Delimiting the proteinase (domain); Deletion/truncation analysis; Screening for proteolytic activity using translation systems in vitro
4 Bacterial expressionExpression of inactive proteinase; Affinity 'tagging'; Insolubility of expressed proteinases; 5 Substrate specificity; 6 Artificial 'reporter' polyproteins; References; 5 Detection and analysis of host gene targets for oncogenic retroviruses; 1 Introduction; 2 Insertional mutagenesis; Analysis of virus integration patterns; Cloning of virus integration sites; Analysis of host DNA flanking the provirus integration sites; Location of genes at integration sites; 3 Transduction of virus oncogenes; Properties of transducing viruses; Biological methods of detection
Summary RNA Viruses: A Practical Approach provides a broad treatment of the principles and practice of RNA virus research to ensure the widest possible audience. RNA Viruses: A Practical Approach will be of interest to all laboratories involved in virus culture, including both academic and industrial research groups and public health laboratories
Bibliography Includes bibliographical references and index
Notes Print version record
Subject RNA viruses -- Research -- Methodology
Viral genomes.
RNA Viruses -- genetics
RNA Viruses -- isolation & purification
Genome, Viral
SCIENCE -- Life Sciences -- Biology.
SCIENCE -- Life Sciences -- Microbiology.
Viral genomes
RNS-Viren
Virologie.
Virus à ARN.
Form Electronic book
Author Cann, Alan
LC no. 99057577
ISBN 0585484295
9780585484297