Title Retinoid protocols / edited by Christopher P.F. Redfern

Published Totowa, N.J. : Humana Press, ©1998

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xvi, 434 pages) : illustrations Series Methods in molecular biology ; v. 89 Methods in molecular biology (Clifton, N.J.) ; v. 89 Contents Properties of retinoids : structure, handling, and preparation / Arun B. Barua, Harold C. Furr -- Quantitative analyses of naturally occurring retinoids / Joseph L. Napoli, Ronald L. Horst -- Detection and measurement of retinoic acid production by isolated tissues using retinoic acid-sensitive reporter cell lines / Michael Wagner -- Immunohistochemistry for CRBPs and CRABPs / Ulf Eriksson, Anne-Lee Gustafsson -- Whole-mount in situ hybridization of mouse embryos exposed to teratogenic levels of retinoic acid / Sally D. Lyn -- Reverse transcriptase-polymerase chain reaction (RT-PCR) for cellular retinoid-binding proteins / Andrew D. Loughney, Christopher P. Redfern -- Methods for producing recombinant human cellular retinaldehyde-binding protein / John W. Crabb, Yang Chen, Steve Goldflam, Karen West, James Kapron -- Expression and purification of CRABPs from E. coli / Joseph L. Napoli, Zuzana Sperkova, Marek Sperka, Paul D. Fiorella -- Purification and fluorescent titration of cellular retinol-binding protein / Giorgio Malpeli, Claudia Folli, Davide Cavazzini, Giovanni Sartori, Rodolfo Berni -- Fluorometric titration of the CRABPs / Andrew W. Norris, Ellen Li -- Expression and mutagenesis of retinol-binding protein / Manickavasagam Sundaram, Asipu Sivaprasadarao, John B. Findlay -- Interactions of retinol-binding protein with transthyretin and its receptor / Asipu Sivaprasadarao, Manickavasagam Sundaram, John B. Findlay -- Detection of conformational changes in cellular retinoid-binding proteins by limited proteolysis / Robert S. Jamison, Marcia E. Newcomer, David E. Ong -- Measurement of rates of dissociation of retinoids from the interphotoreceptor retinoid-binding protein / Noa Noy -- Use of antisense oligonucleotides to study the role of CRABPs in retinoic acid-induced gene expression / Paul Nugent, Robert M. Greene -- Preparation of polyclonal antibodies to retinoid receptors / Jacqueline A. Dyck, Vickie J. LaMorte -- Detection of RARs and RXRs in cells and tissues using specific ligand-binding assays and ligand-binding lmmunoprecipitation techniques / Elizabeth A. Allegretto -- Nonisotopic in situ hybridization for the detection of nuclear retinoid receptor transcripts in tissue sections / Xiaochun Xu, Reuben Lotan -- In situ hybridization with ³⁵S-labeled probes for retinoid receptors / Karen Niederreither, Pascal Dollé -- Isolation of retinoid receptors from manimalian cells / Ann K. Daly, Christopher P. Redfern -- Analysis of retinoid receptor phosphorylation / Ali Tahayato, Christophe Rachez, Pierre Formstecher, Philippe Lefebvre -- Photoaffinity labeling of RARs and mapping of labeled sites by an endoproteinase combination technique / Yuichi Hashimoto, Toru Sasaki -- PCR cloning of N-terminal RAR Isoforms and APL-associated PLZF-RAR[alpha] fusion proteins / Arthur Zelent -- RT-PCR in diagnosis and disease monitoring of acute promyelocytic leukemia (APL) / David Grimwade, Stephen Langabeer, Kathy Howe, Ellen Solomon -- A two-hybrid protein interaction system to identify factors that interact with retinoid and vitamin D receptors / Paul N. MacDonald -- Gel-shift analysis and identification of RXREs and RAREs by PCR-based selection / Myriam I. Baes, Peter E. Declercq -- Identification and cloning of RA-regulated genes by mRNA-differential display / Jay A. White, Martin Petkovich -- Gene targeting of retinoid receptors / David Lohnes Summary Christopher Redfern brings together in Retinoid Protocols a comprehensive collection of key biochemical and molecular methods for retinoid research. These easily reproducible techniques range from methods of handling and analyzing retinoids to advanced protocols for gene targeting, from binding protein function in transgenic animals to the study of nuclear retinoid receptors. In addition, the book makes accessible to all retinoid researchers such advanced techniques as cloning via RT-PCR, fluorimetry methods, recombinant protein purification and characterization, the use of antisense oligonucleotides to study the role of CRABPs, immunological and in situ hybridization methods, photoaffinity labeling, gel-shift analysis, and differential display. Many of the chapters in Retinoid Protocols represent landmarks in retinoid methodology because they give step-by-step instructions for techniques central to understanding cellular control by retinoids. These indispensable techniques will be fundamental for all those working with retinoids, and so will play a key role in the development of new drugs and therapies for treating human disease Bibliography Includes bibliographical references and index Notes English Print version record In Springer Protocols Subject Retinoids -- Laboratory manuals Retinoids -- Analysis -- Laboratory manuals Genetics -- Technique. Retinoids. Retinoids -- chemistry Retinoids -- pharmacology Genetic Techniques Receptors, Retinoic Acid Retinoids Genetics -- Technique Retinoids Retinoids -- Analysis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Redfern, Christopher P. F. ISBN 9781592595730 1592595731 1280836881 9781280836886 9786610836888 6610836884

  Permalink