| Description |
1 online resource (xi, 270 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 103 |
|
Methods in molecular biology (Clifton, N.J.) ; volume 103.
|
| Contents |
Introduction to Pichia pastoris / David R. Higgins, James M. Cregg -- Classical genetic manipulation / James M. Cregg, Shigang Shen, Monique Johnson, Hans R. Waterham -- Transformation / James M. Cregg, Kimberly A. Russell -- Small vectors for expression based on dominant drug resistance with direct multicopy selection / David R. Higgins, Katie Busser, John Comiskey, Peter S. Whittier, Thomas J. Purcell, James P. Hoeffler -- The generation of multicopy recombinant strains / Mike Romanos, Carol Scorer, Koti Sreekrishna, Jeff Clare -- Isolation of nucleic acids / David J. Miles, Katie Busser, Christine Stalder, David R. Higgins -- Generation of protease-deficient strains and their use in heterologous protein expression / Martin A. Gleeson, Christopher E. White, David P. Meininger, Elizabeth A. Komives -- Glycosylation profiling of heterologous proteins / José A. Cremata, Raquel Montesino, Omar Quintero, Rossana García -- High cell-density fermentation / Jayne Stratton, Vijay Chiruvolu, Michael Meagher -- Use of Pichia pastoris as a model eukaryotic system : peroxisome biogenesis / Klaas Nico Faber, Ype Elgersma, John A. Heyman, Antonius Koller, Georg H. Lüers, William M. Nuttley, Stanley R. Terlecky, Thibaut J. Wenzel, Suresh Subramani -- Secretion of recombinant human insulin-like growth factor I (IGF-I) / Russell A. Brierley -- Secretion of scFv antibody fragments / Hermann Gram, Rita Schmitz, Rüdiger Ridder -- Expression of tetanus toxin fragment C / Jeff Clare, Koti Sreekrishna, Mike Romanos -- Expression of EGF and HIV envelope glycoprotein / Jeff Clare, Carol Scorer, Rich Buckholz, Mike Romanos -- Expression of an integral membrane protein, the 5HT5A receptor / H. Markus Weiss, Winfried Haase, Helmut Reiländer -- Localization of the myc-Tagged 5HT5A receptor by immunogold staining of ultrathin sections / Winfried Haase, H. Markus Weiss, Helmut Reiländer -- Foreign proteins expressed in P. pastoris |
| Summary |
The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Pichia pastoris.
|
|
Yeast fungi -- Biotechnology.
|
|
Genetic vectors.
|
|
Pichia.
|
|
Bioengineering.
|
|
Biotechnology.
|
|
Pichia
|
|
Biotechnology
|
|
Genetic Vectors
|
|
Saccharomycetales -- genetics
|
|
bioengineering.
|
|
MEDICAL -- Microbiology.
|
|
Pichia
|
|
Biotechnology
|
|
Bioengineering
|
|
Genetic vectors
|
|
Pichia pastoris
|
|
Yeast fungi -- Biotechnology
|
|
Methylotrofe bacteriën.
|
|
Gist.
|
|
Wetenschappelijke technieken.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Higgins, David R. (David Robert), 1958-
|
|
Cregg, James M
|
| LC no. |
98006428 |
| ISBN |
9781592595785 |
|
0896034216 |
|
9780896034211 |
|
1592595782 |
|
1280843071 |
|
9781280843075 |
|
9786610843077 |
|
6610843074 |
|
