| Description |
1 online resource (xiv, 385 pages) : illustrations |
| Series |
Methods in molecular medicine ; 38 |
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Methods in molecular medicine ; 38.
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| Contents |
1. Understanding Aging / Bernard L. Strehler -- pt. I. Study of Cell Senescence and Cell Death -- 2. Use of the Fibroblast Model in the Study of Cellular Senescence / Vincent J. Cristofalo, Craig Volker and Robert G. Allen -- 3. Human T-Cell Clones / Graham Pawelec -- 4. Telomeres and Replicative Senescence / Hector F. Valenzuela and Rita B. Effros -- 5. Detection of Molecular Events During Apoptosis by Flow Cytometry / Ruaidhri J. Carmody, Ana P. Costa-Pereira, Sharon L. McKenna, and Tom G. Cotter -- 6. Raf-1 Protein Kinase Activity in T Cells from Aged Mice / Christopher J. Kirk and Richard A. Miller -- 7. Identification of Differentially Expressed Genes in Young and Senescent T Cells / Andrea Engel, Mahdi Adibzadeh and Graham Pawelec -- pt. II. Age-Related Changes to Xenobiotic Metabolizing Enzyme Systems -- 8. Xenobiotic-Metabolizing Enzyme Systems and Aging / Christopher R. Barnett and Costas Ioannides -- pt. III. Age-Related Changes to Defense Systems Against Biomolecule Damage -- 9. Assessing Age-Related Changes in Antioxidant Status: The FRASC Assay for Total Antioxidant Power and Ascorbic Acid Concentration in Biological Fluids / Iris F.F. Benzie and John J. Strain -- 10. Measurement of DNA Damage and Repair Capacity as a Function of Age Using the Comet Assay / Peter H. Clingen, Jillian E. Lowe and Michael H.L. Green -- 11. Measurement of DNA Damage and Repair in Human White Blood Cells by an Immunochemical Assay / Govert P. van der Schans -- pt. IV. Age-Related Changes to Cellular Biomolecules -- 12. Measurement of 8-Oxo-deoxyguanosine in Lymphocytes, Cultured Cells, and Tissue Samples by HPLC with Electrochemical Detection / Sharon G. Wood, Catherine M. Gedik, Nicholas J. Vaughan, and Andrew R. Collins -- 13. Mutation and the Aging Process: Mutant Frequency at the HPRT Gene Locus as a Function of Age in Humans / Yvonne A. Barnett and Christopher R. Barnett -- 14. Somatic Mutations and Aging: Methods for Molecular Analysis of HPRT Mutations / Sai-Mei Hou -- 15. Assessment of Susceptibility of Low-Density Lipoprotein to Oxidation / Jane McEneny and Ian S. Young -- 16. Measurement of Pentosidine in Biological Samples / Jesus R. Requena, David L. Price, Suzanne R. Thorpe, and John W. Baynes -- pt. V. Mitochondrial Morphology, Function and Mutation with Age -- 17. Causes and Consequences of Damage to Mitochondria: Morphological Aspects / Jaime Miquel and Carlo Bertoni-Freddari -- 18. Causes and Consequences of Damage to Mitochondria: Study of Functional Aspects by Flow Cytometry / Federico V. Pallardo, Juan Sastre, Jaime Miquel, and José Viña -- 19. Analysis of Mitochondrial DNA Mutations: Deletions / Robert W. Taylor, Theresa M. Wardell, Emma L. Blakely, Gillian M. Borthwick, Elizabeth J. Brierley, and Douglass M. Turnbull -- 20. Analysis of Mitochondrial DNA Mutations: Point Mutations / Robert W. Taylor, Richard M. Andrews, Patrick F. Chinnery, and Douglass M. Turnbull -- pt. VI. Immune System and Aging -- 21. Assessment of T-Cell Function in the Aged: T-Cell Proliferative and T-Cell Adherence Assays / Ian Beckman -- 22. Dendritic Cells in Old Age / Beatrix Grubeck-Loebenstein, Maria Saurwein-Teissl and Nikolaus Romani -- 23. Age-Related Alterations to Natural Killer Cell Function / Erminia Mariani, Corona Alonso and Rafael Solana -- 24. Immunogenetics and Life-Span: HLA / Derek Middleton, Martin D. Curran and Fionnuala Williams -- pt. VII. Case Studies -- 25. Dietary Restriction and Life-Span Extension / Byung Pal Yu -- 26. Use of Genetically Engineered Mice in Aging Research / Julie K. Andersen |
| Summary |
With rapidly rising life expectancies and a general lack of understanding about the aging process, the need to treat geriatric diseases is becoming an ever more significant private and public health issue. In Aging Methods and Protocols, Yvonne and Christopher Barnett and a team of recognized international experts detail key biochemical, analytical, and molecular techniques for the investigation of aging at the cellular, tissue, organ, and whole system levels. These cutting-edge methods address a wide range of research needs, from uncovering the factors associated with cell senescence and death, to exploring alterations in the body's ability both to metabolize xenobiotics, and to defend itself against biomolecular damage. State-of-the-art protocols also measure the morphological, functional, and molecular changes that accumulate within mitochrondria over time, and permit the genetic and functional characteristics of the immune system to be determined. Two important case studies examine the role of dietary restriction on life span extension and the use of transgenic animals in the molecular analysis of aging. Wide ranging and highly practical, Aging Methods and Protocols provides today's molecular gerontologists, pharmacologists, and clinical investigators with a gold-standard collection of readily reproducible techniques for identifying those key cellular and molecular processes that might one day make it possible to regulate the aging process |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Aging -- Molecular aspects -- Laboratory manuals
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Aging.
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Research.
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Methodology.
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Science.
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Physical sciences.
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Research Design
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Aging
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Growth and Development
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Research
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Methods
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Science
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Physiological Phenomena
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Investigative Techniques
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Natural Science Disciplines
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Phenomena and Processes
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Disciplines and Occupations
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research (function)
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methodology.
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sciences (philosophy)
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physical sciences.
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science (modern discipline)
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SCIENCE -- Life Sciences -- Developmental Biology.
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Science
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Research
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Physical sciences
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Methodology
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Aging
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Aging -- Molecular aspects
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Barnett, Yvonne A.
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Barnett, Christopher R.
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| ISBN |
9781592590704 |
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1592590705 |
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0585344183 |
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9780585344188 |
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1280820616 |
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9781280820618 |
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9786610820610 |
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6610820619 |
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