| Description |
xx, 339 pages : illustrations ; 24 cm |
| Series |
The practical approach series ; 173 |
|
Practical approach series ; 173
|
| Contents |
1. Homogenization of tissues and cells / John M. Graham -- 2. Isolation of subcellular fractions / Richard H. Hinton and Barbara M. Mullock -- 3. Isolation and characterization of nuclei and nuclear subfractions / David Rickwood, Anthea Messent and Dipak Patel -- 4. Subcellular fractionation of mitochondria / J. E. Rice and J. G. Lindsay -- 5. Isolation and characterization of peroxisomes / A. Volkl, E. Baumgart and H. D. Fahimi -- 6. Lysosomes and endocytosis / Tor Gjoen, Trond Olav Berg and Trond Berg -- 7. The membranes of the secretory and exocytic pathways / John M. Graham -- 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells / Anthony L. Moore and David G. Whitehouse -- 9. Ribosomes and polysomes / Ulrich A. Bommer, Nils Burkhardt and Ralf Junemann [et al.] -- 10. Electron microscopy of organelles / Nigel James -- A1. Core list of suppliers |
| Summary |
Many investigations into cell structure and function require the isolation of a particular subcellular particle. Subcellular Fractionation covers the subject comprehensively, describing in detail the wide range of separation techniques and characterization procedures for all the major subcellular organelles: nuclei, mitochondria, chloroplasts, peroxisomes, and the membrane systems of the exocytic and endocytic pathways. Importantly, the text also describes the isolation of chromosomes, nucleoli and nucleoprotein complexes, and key procedures related to the analysis of these particles, such as the labelling of ligands, kinetic analysis of their internalization, and electron microscopy |
| Analysis |
fractions nuclear subfractions nuclei mitochondria peroxisomes lysoso mes endocytosis secretory pathways ribosomes polysomes organelles |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Cell fractionation -- Handbooks, manuals, etc.
|
|
Cell fractionation -- Laboratory manuals.
|
|
Subcellular fractionation -- Handbooks, manuals, etc.
|
|
Subcellular fractionation -- Laboratory manuals.
|
|
Subcellular Fractions -- analysis
|
|
Subcellular Fractions -- methods
|
| Genre/Form |
Handbooks and manuals.
|
| Author |
Graham, J. M. (John M.), 1943-
|
|
Rickwood, D. (David)
|
| LC no. |
97179028 |
| ISBN |
0199634947 (paperback) |
|
0199634955 |
|
