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Title Subcellular fractionation : a practical approach / edited by J.M. Graham and D. Rickwood
Published Oxford ; New York : IRL Press at Oxford University Press, [1997]
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Description 1 online resource (xx, 339 pages) : illustrations
Series The practical approach series ; 173
Practical approach series ; 173
Contents Machine generated contents note: 1. Homogenization of tissues and cells / John M. Graham -- 2. Isolation of subcellular fractions / Barbara M. Mullock / Richard H. Hinton -- 3. Isolation and characterization of nuclei and nuclear subfractions / Anthea Messent / David Rickwood / Dipak Patel -- 4. Subcellular fractionation of mitochondria / J. E. Rice / J. G. Lindsay -- 5. Isolation and characterization of peroxisomes / E. Baumgart / H. D. Fahimi / A. Volkl -- 6. Lysosomes and endocytosis / Trond Berg / Tor Gjoen / Trond Olav Berg -- 7. membranes of the secretory and exocytic pathways / John M. Graham -- 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells / Anthony L. Moore / David G. Whitehouse -- 9. Ribosomes and polysomes / Ralf Junemann / Nils Burkhardt / Ulrich A. Bommer -- 10. Electron microscopy of organelles / Nigel James -- A1. Core list of suppliers
Summary "Many investigations into cell structure and function require the isolation of a particular subcellular particle. Subcellular Fractionation covers the subject comprehensively, describing in detail the wide range of separation techniques and characterization procedures for all the major subcellular organelles: nuclei, mitochondria, chloroplasts, peroxisomes, and the membrane systems of the exocytic and endocytic pathways. Importantly, the text also describes the isolation of chromosomes, nucleoli and nucleoprotein complexes, and key procedures related to the analysis of these particles, such as the labelling of ligands, kinetic analysis of their internalization, and electron microscopy." --Book Jacket
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Subcellular fractionation -- Handbooks, manuals, etc.
Subcellular fractionation -- Laboratory manuals.
Subcellular Fractions.
Subcellular Fractions -- analysis
Genre/Form methods
Handbooks and manuals.
Laboratory manuals.
Handbooks and manuals.
Laboratory manuals.
Form Electronic book
Author Graham, J. M. (John M.), 1943-
Rickwood, D. (David)
ISBN 0585483922 (electronic bk.)
9780585483924 (electronic bk.)