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E-book

Title Tissue morphogenesis : methods and protocols / edited by Celeste M. Nelson
Published New York, NY : Humana Press, [2015]
©2015

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Description 1 online resource (xii, 351 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; 1189
Methods in molecular biology (Clifton, N.J.) ; v. 1189. 1064-3745
Contents Probing Regional Mechanical Properties of Embryonic Tissue Using Microindentation and Optical Coherence Tomography -- Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV -- Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis -- Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis -- Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos -- Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly -- Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis -- A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis -- Active Cell and ECM Movements During Development -- 3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion -- Culture of Mouse Embryonic Foregut Explants -- Investigating human vascular tube morphogenesis and maturation using endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices -- Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues -- Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin Networks in the Early Developing Drosophila Embryo -- UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in vivo and ex vivo -- Measurement of Intercellular Cohesion by Tissue Surface Tension -- Quail-Chick Chimeras and Eye Development -- Studying Epithelial Morphogenesis in Dictyostelium -- Primary Cell Cultures of Regenerating Holothurian Tissues -- Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis Using CompuCell3D or Virtual Leaf -- Simulating Tissue Morphogenesis and Signaling -- Elasticity-Based Targeted Growth Models of Morphogenesis
Summary Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational for the study of tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis, and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis
Analysis morfogenese
morphogenesis
celbiologie
cellular biology
moleculaire genetica
molecular genetics
weefsels
tissues
Ontogeny, Embryology
Ontogenie, embryologie
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed October 13, 2014)
Subject Tissue remodeling -- Laboratory manuals
Morphogenesis.
Morphogenesis
Tissues -- growth & development
Cellular biology (cytology)
Medical genetics.
Medical -- Genetics.
Science -- Life Sciences -- Biology -- Molecular Biology.
Morphogenesis
Tissue remodeling
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Nelson, Celeste M., editor.
ISBN 9781493911646
1493911643
1493911635
9781493911639