Title Bacterial transcriptional control : methods and protocols / edited by Irina Artsimovitch, Thomas J. Santangelo

Published New York, NY : Humana Press, [2015] ©2015

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Description 1 online resource (xi, 342 pages) : illustrations (some color) Series Methods in Molecular Biology, 1064-3745 ; 1276 Methods in molecular biology (Clifton, N.J.) ; v. 1276. 1064-3745 Contents Mapping the Escherichia coli transcription elongation complex with exonuclease III / Zhaokun Liu and Irina Artsimovitch -- Purification of bacterial RNA polymerase : tools and protocols / Vladimir Svetlov and Irina Artsimovitch -- Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues / Anssi M. Malinen, Matti Turtola, and Georgiy A. Belogurov -- In vitro and in vivo methodologies for studying the sigma 54-dependent transcription / Martin Buck [and others] -- Methods for the assembly and analysis of in vitro transcription-coupled-to-translation systems / Daniel Castro-Roa and Nikolay Zenkin -- Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and staudinger-bertozzi ligation / Anirban Chakraborty [and others] -- Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts / Jian Li and David S. Gilmour -- Direct competition assay for transcription fidelity / Lucyna Lubkowska and Maria L. Kireeva -- Single-stranded DNA aptamers for functional probing of bacterial RNA polymerase / Danil Pupov and Andrey Kulbachinskiy -- Biochemical analysis of transcription termination by RNA polymerase III from yeast Saccharomyces cerevisiae / Aneeshkumar G. Arimbasseri and Richard J. Maraia -- Use of RNA polymerase molecular beacon assay to measure RNA polymerase interactions with model promoter fragments / Vladimir Mekler and Konstantin Severinov -- Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends / Irina O. Vvedenskaya, Seth R. Goldman, and Bryce E. Nickels -- In situ footprinting of E. coli transcription elongation complex with chloroacetaldehyde / A. Rachid Rahmouni and Christine Mosrin-Huaman -- Using solutes and kinetics to probe large conformational changes in the steps of transcription initiation / Emily F. Ruff, Wayne S. Kontur, and M. Thomas Record Jr. -- Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro / Alexandra M. Gehring and Thomas J. Santangelo -- Purification of active RNA polymerase I from yeast / Francis Dean Appling and David Alan Schneider -- Transcription in archaea : preparation of Methanocaldococcus jannaschii transcription machinery / Katherine Smollett, Fabian Blombach, and Finn Werner -- Transcription in archaea : in vitro transcription assays for mjRNAP / Katherine Smollett, Fabian Blombach, and Finn Werner -- Experimental analysis of hFACT action during Pol II transcription in vitro / Fu-Kai Hsieh, Olga I. Kulaeva, and Vasily M. Studitsky -- ChIP-Seq for genome-scale analysis of bacterial DNA- binding proteins / Richard P. Bonocora and Joseph T. Wade Summary This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future Analysis bacteria microbiologie microbiology bacteriologie bacteriology genetische modificatie genetic engineering Bacteriology Bacteriologie Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed February 20, 2015) Subject RNA polymerases -- Laboratory manuals Genetic transcription -- Laboratory manuals RNA polymerases. Genetic transcription. DNA-Directed RNA Polymerases Bacterial Proteins -- genetics Transcription, Genetic Genetic transcription RNA polymerases Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Artsimovitch, Irina, editor Santangelo, Thomas J., editor ISBN 9781493923922 1493923927 1493923919 9781493923915

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