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E-book

Title Gapmers : methods and protocols / edited by Toshifumi Yokota, Rika Maruyama
Published New York, NY : Humana Press, [2020]

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Description 1 online resource
Series Methods in molecular biology ; 2176
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; 2176.
Springer protocols (Series)
Summary This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated RNA knockdown for basic research and therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gapmers: Methods and Protocols aims to provide diverse applications of gapmers along with protocols that will assist readers in moving the frontier
Notes Includes index
Online resource; title from digital title page (viewed on October 16, 2020)
Subject Triple-helix-forming oligonucleotides -- Laboratory manuals
Oligonucleotides, Antisense
Medical genetics.
Medical -- Genetics.
Triple-helix-forming oligonucleotides
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Yokota, Toshifumi, editor.
Maruyama, Rika, editor.
ISBN 9781071607718
1071607715