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Title Tumor profiling : methods and protocols / edited by Sarah S. Murray
Published New York, NY : Humana Press : Springer, [2019]
©2019

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Description 1 online resource (xi, 268 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1908
Methods in molecular biology (Clifton, N.J.) ; v. 1908. 1064-3745
Contents Amplicon-based targeted next-generation sequencing of formalin-fixed, paraffin-embedded tissue / Eric Strengman, Francoise A.S. Barendrecht-Smouter, Carmen de Voijs, Paula de Vree, Isaac J. Nijman, and Wendy W.J. de Leng -- Library preparation using FFPE-derived tumor DNA for high-throughput hybridization-based targeted or exome sequencing / John A. Thorson and Sarah S. Murray -- Bioinformatics basics for high-throughput hybridization-based targeted DNA sequencing from FFPE-derived tumor specimens : from reads to variants / Shulei Sun and Sarah S. Murray -- Annotation of variant data from high-throughput DNA sequencing from tumor specimens : filtering strategies to identify driver mutations / Shulei Sun, John A. Thorson, and Sarah S. Murray -- Biological interpretation of complex genomic data / Kathleen M. Fisch -- Clinical validation of targeted solid tumor profiling / Guy Froyen and Brigitte Maes -- Whole-genome single nucleotide polymorphism microarray for copy number and loss of heterozygosity analysis in tumors / Ross Rowsey, Iya Znoyko, and Daynna J. Wolff -- Genome-wide copy number variation detection using NGS : data analysis and interpretation / Wei Shen, Philippe Szankasi, Jacob Durtschi, Todd W. Kelley, and Xinjie Xu -- Overview of fusion detection strategies using next-generation sequencing / Jan Schro·der, Amit Kumar, and Stephen Q. Wong -- Clinical application of fusion gene detection using next-generation sequencing and the nanostring technology / Anna Karlsson and Johan Staaf -- Pipeline for integrated microarray expression normalization tool kit (PIMENTo) for tumor microarray profiling experiments / Thomas Nash, Matthew Huff, W. Bailey Glen Jr., and Gary Hardiman -- Molecular profiling of RNA tumors using high-throughput RNA sequencing : overview of library preparation methods / Sean M. Courtney, Willian A. da Silveira, E. Starr Hazard, and Gary Hardiman -- Molecular profiling of RNA tumors using high-throughput RNA sequencing : from raw data to systems level analyses / Willian A. da Silveira, E. Starr Hazard, Dongjun Chung, and Gary Hardiman -- Methylation analysis using microarrays : analysis and interpretation / Teresia Kling and Helena Careþn -- High-throughput targeted repeat element bisulfite sequencing (HT-TREBS) / Arundhati Bakshi, Muhammad B. Ekram, and Joomyeong Kim -- Pipeline for ctDNA detection following primary tumor profiling using a cancer-related gene sequencing panel / Satoshi S. Nishizuka, Kei A. Sato, and Tsuyoshi Hachiya -- Single-cell analysis of circulating tumor cells / Jana- A. Thiele, Pavel Pitule, James Hicks, and Peter Kuhn
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed January 16, 2019)
Subject Tumors -- Genetic aspects -- Laboratory manuals
Neoplasms
Tumors -- Genetic aspects
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Murray, Sarah S., editor
ISBN 9781493990047
1493990047