| Description |
1 online resource (xix, 396 pages) : illustrations |
| Series |
Methods in molecular biology ; 269 |
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Methods in molecular biology (Clifton, N.J.) ; v. 269
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| Contents |
Working safely with Vaccinia virus : laboratory technique and the role of Vaccinia vaccination / Stuart N. Isaacs -- Construction and isolation of recombinant Vaccinia virus using genetic markers / Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco -- Construction of recombinant Vaccinia virus : cloning into the thymidine kinase locus / Chelsea M. Byrd and Dennis E. Hruby -- Transient and inducible expression of Vaccinia/T7 recombinant viruses / Mohamed R. Mohamed and Edward G. Niles -- Construction of recombinant Vaccinia viruses using leporipoxvirus catalyzed recombination and reactivation of orthopoxvirus DNA / Xiao-Dan Yao and David H. Evans -- Construction of CDNA libraries in Vaccinia virus / Ernest S. Smith, Shuying Shi, and Maurice Zauderer -- Construction and isolation of recombinant MVA / Caroline Staib, Ingo Drexler, and Gerd Sutter -- Growing poxviruses and determining virus titer / Girish J. Kotwal and Melissa-Rose Abrahams -- Rapid preparation of vaccinia virus DNA template for analysis and cloning by PCR / Rachel L. Roper -- Orthopoxvirus diagnostics / Hermann Meyer, Inger K. Damon, and Joseph J. Esposito -- An in vitro transcription system for studying vaccinia virus early genes / Steven S. Broyles and Marcia Kremer -- An in vitro transcription system for studying vaccinia virus late genes / Cynthia F. Wright -- Studying vaccinia virus RNA processing in vitro / Paul D. Gershon -- Methods for analysis of poxvirus DNA replication / Paula Traktman and Kathleen Boyle -- Studying the binding and entry of the intracellular and extracellular enveloped forms of vaccinia virus / Mansun Law and Geoffrey L. Smith -- Pox, dyes, and videotape: Making movies of GFP-labeled vaccinia virus / Brian M. Ward -- Interaction analysis of viral cytokine-binding proteins using surface plasmon resistance / Bruce T. Seet and Grant McFadden -- Monitoring of human immunological responses to vaccinia virus / Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll -- Vaccinia virus as a tool for immunologic studies / Nia Tatsis, Gomathinayagam Sinna bathamby, and Laurence C. Eisenlohr -- Mouse models for studying orthopoxvirus respiratory infections / Jill Schriewer, R. Mark Buller, and Gelita Owens -- Viral glycoprotein-mediated cell fusion assays using Vaccinia virus vectors / Katharine N. Bossart and Christopher C. Broder -- Use of dual recombinant Vaccinia virus vectors to assay viral glycoprotein-mediated fusion with transfection-resistant primary cell targets / Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman -- Poxvirus bioinformatics / Chris Upton -- Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens / Nadja V. Melquiot and Joachim J. Bugert |
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Construction and isolation of recombination MVA / Caroline Staib, Ingo Drexler, and Gerd Sutter -- Growing poxviruses and determining virus titer / Girish J. Kotwal and Melissa Abraham -- Rapid preparation of Vaccinia virus DNA template for analysis and cloning by PCR / Rachel L. Roper -- Orthopoxvirus diagnostics / Hermann Meyer, Inger K. Damon, and Joseph J. Esposito -- An in vitro transcription system for studying Vaccinia virus early genes / Steven S. Broyles and Marcia Kremer -- An in vitro transcription system for studying Vaccinia virus late genes / Cynthia F. Wright -- Studying Vaccinia virus RNA processing in vitro / Paul D. Gershon -- Methods for analysis of poxvirus DNA replication / Paula Traktman and Kathleen Boyle -- Viral glycoprotein-mediated cell fusion assays using Vaccinia virus vectors / Katharine N. Bossart and Christopher C. Broder -- Use of dual recombinant Vaccinia virus vectors to assay viral glycoprotein-mediated fusion with transfection-resistant primary cell targets / Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman -- Poxvirus bioinformatics / Chris Upton -- Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens / Nadja V. Melquiot and Joachim J. Bugert |
| Summary |
Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols, internationally recognized experts and leaders in poxvirology describe in step-by-step detail the successful techniques they have perfected to study and work with vaccinia virus and other poxviruses. They offer cutting-edge techniques for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxvirus-the latter critical to the important role of vaccinia virus in smallpox vaccination and its potential role as a vaccine vector directed against infectious agents and cancer. The protocols presented follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and timely, Vaccinia Virus and Poxvirology: Methods and Protocols offers virologists and vaccinologists an unequaled source of proven techniques for understanding poxviruses and developing defenses against their use as bioterrorist agents |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Vaccinia -- Laboratory manuals
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Recombinant poxviruses -- Laboratory manuals
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DNA replication.
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Viruses -- Reproduction.
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Vaccinia virus -- genetics
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DNA Replication
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Molecular Biology -- methods
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Poxviridae -- genetics
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Vaccinia virus -- immunology
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Virus Replication
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SCIENCE -- Life Sciences -- Biology.
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SCIENCE -- Life Sciences -- Microbiology.
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Viruses -- Reproduction
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DNA replication
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Recombinant poxviruses
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Vaccinia
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Isaacs, Stuart Neal.
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| ISBN |
1588292290 |
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9781588292292 |
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9781592597895 |
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1592597890 |
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1280360143 |
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9781280360145 |
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9786610360147 |
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6610360146 |
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