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E-book

Title VEGF signaling : methods and protocols / edited by Lorna Fiedler
Published New York, NY : Humana Press, [2015]
©2015

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Description 1 online resource (x, 225 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; v. 1332
Methods in molecular biology (Clifton, N.J.) ; v. 1332, 1064-3745
Contents VEGF splicing and the role of VEGF splice variants: From physiological-pathological conditions to specific pre-mRNA splicing -- Detection and quantification of VEGF isoforms by ELISA -- Quantitation of circulating neuropilin-1 in human, monkey, mouse, and rat sera by ELISA -- Detection and quantification of vascular endothelial growth factor receptor tyrosine kinases in primary human endothelial cells -- Induction of VEGF secretion in cardiomyocytes by mechanical stretch -- Chromatin immunoprecipitation assay: Examining the interaction of NFkB with the VEGF promoter -- An overview of vegf-mediated signal transduction -- Identification of receptor tyrosine kinase inhibitors using cell surface biotinylation and affinity isolation -- Analysis of VEGF-mediated ERK5 activity in endothelial cells -- In vitro angiogenesis assays -- Chemotactic migration of endothelial cells towards VEGF-A165 -- Vasculogenesis and angiogenesis in VEGF receptor-1 deficient mice -- The embryonic mouse hindbrain and postnatal retina as in vivo models to study angiogenesis -- VEGF gene transfer to the utero-placental circulation of pregnant guinea pigs to enhance fetal growth -- VEGF gene transfer to the utero-placental circulation of pregnant sheep to enhance fetal growth -- Generation of targeted mutations in zebrafish using the CRISPR/cas system
Summary This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of this book is that each chapter not only contains methodological detail rarely seen in other literature, but also contains a section on key notes and troubleshooting advice. Part I focuses on quantification of specific VEGF and VEGF receptor isoforms, beginning with a summary on VEGF splice variants, their function, and their regulation. Part II relates to the study of VEGF dependent signaling in vitro, starting with a comprehensive overview of VEGF-mediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, VEFG Signaling: Methods and Protocols is a useful tool for researching and understanding the basic biology of VEGF signaling, and translating it into the clinic
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed August 28, 2015)
Subject Vascular endothelial growth factors -- Laboratory manuals
Cellular signal transduction -- Laboratory manuals
Vascular endothelial growth factors.
Vascular Endothelial Growth Factors
Mechanotransduction, Cellular
Biochemistry.
Science -- Life Sciences -- Biochemistry.
Cellular signal transduction
Vascular endothelial growth factors
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Fiedler, Lorna, editor.
ISBN 9781493929177
1493929178
149392916X
9781493929160