| Description |
1 online resource (xi, 357 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 515 |
|
Springer protocols |
|
Methods in molecular biology (Clifton, N.J.) ; v. 515.
|
|
Springer protocols (Series)
|
| Contents |
1. Using fluorescent proteins to study poxvirus morphogenesis / Brian M. Ward -- 2. Application of reporter gene imaging for studying human embryonic stem cells in living subjects / Kazim H. Narsinh, Feng Cao, and Joseph C. Wu -- 3. Use of a GFP-PML-expressing cell line as a biosensor for human cytomegalovirus infection / Tomonori Ueno and Kiyoko Ogawa-Goto -- 4. Dual-color imaging of tumor angiogenesis / Robert M. Hoffman -- 5. Use of GFP to analyze morphology, connectivity, and function of cells in the central nervous system / Alan R. Harvey [and others] -- 6. Expression of EGFP by adenovirus-mediated gene transfer in the central nervous system / Ryohei Tomioka -- 7. Adenovirus-mediated delivery of short hairpin RNA (shRNA) mediates efficient gene silencing in terminally differentiated cardiac myocytes / Andreas Rinne [and others] -- 8. Microscopic analysis of adenoviral decontamination using GFP adenovirus with comparable sensitivity to flow cytometry / Michael Grusch [and others] -- 9. Production of multicistronic HIV-1 based lentiviral vectors / Nachimuthu Chinnasamy, James Shaffer, and Dhanalakshmi Chinnasamy -- 10. Using an EGFPmeter to evaluate the lentiviral vector production: tricks and traps / Claude Bagnis, Pascal Bailly, and Sylvie Chapel-Fernandes -- 11. Use of a mcrophage-tropic GFP-tagged human immunodeficiency virus type 1 (HIV-1) to study viral reservoirs / Amanda M. Brown -- 12. GFP-lentiviral vectors targeting for neuroAIDS / Yuanan Lu -- 13. Construction of cell-type specific promoter lentiviruses for optically guiding electrophysiological recordings and for targeted gene delivery Aaron M. Jasnow [and others] -- 14. Selective Protein Expression Within the CNS Using Hybrid Lentivirus / Fredrik Gussing [and others] |
|
15. Construction and analysis of alphaherpesviruses expressing green fluorescent protein / Bruce W. Banfield and Gregory A. Bird -- 16. The use of green fluorescent fusion proteins to monitor herpes simplex virus replication / Travis J. Taylor and David M. Knipe -- 17. Modified bovine herpesvirus 1 for protein secretion / Gunther M. Keil -- 18. Identification of viral peptide fragments for vaccine development / Zhanglin Lin, Shuang Li, and Yong Chen -- 19. Use of GFP to investigate expression of plant-derived vaccines / Hong-Ye Li and Mee-Len Chye -- 20. Studies of the role and function of barley stripe mosaic virus encoded proteins in replication and movement using GFP fusions / Sophie Haupt [and others] -- 21. Sendai virus for cancer immunotherapy / Yasuji Ueda, Mamoru Hasegawa, and Yoshikazu Yonemitsu -- 22. Poxvirus tropism for primary human leukocytes and hematopoietic cells / Qigui Yu, Ningjie Hu, and Mario Ostrowski -- 23. Reverse genetics of nipah virus to probe viral pathogenicity / Misako Yoneda [and others] -- 24. Baculovirus-mediated gene transfer into mesenchymal stem cells / Chien-Tai Tsai, Ching-Kuang Chuang, and Yu-Chen Hu |
| Summary |
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein |
| Analysis |
eiwitten |
|
proteins |
|
virussen |
|
viruses |
|
virologie |
|
virology |
|
vectoren |
|
vectors |
|
methodologie |
|
methodology |
|
green fluorescent protein |
|
protocollen |
|
protocols |
|
Virology |
|
Virologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Green fluorescent protein -- Laboratory manuals
|
|
Viruses -- Research -- Methodology
|
|
Viruses.
|
|
Virology -- Research -- Methodology
|
|
Green Fluorescent Proteins -- analysis
|
|
Viruses
|
|
Viruses
|
|
Green fluorescent protein
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hicks, Barry W
|
| ISBN |
9781597455596 |
|
1597455598 |
|
