| Description |
1 online resource (xv, 669 pages) : illustrations |
| Series |
Springer protocols |
|
Springer protocols (Series)
|
| Contents |
Patent and licensing issues in transgenic technology / Karen S. Canady -- Global resources : including gene trapped ES cell clones -- is your gene already knocked out / Lluis Montoliu -- Designing transgenes for optimal expression / Eduardo Molto, Cristina Vicente-Garcıa, and Lluis Montoliu -- Gene targeting vector design for embryonic stem cell modifications / Thomas L. Saunders -- Transgenic production benchmarks / Thomas J. Fielder and Lluis Montoliu -- Generation of transgenic mice by pronuclear microinjection / Katja Becker and Boris Jerchow -- Generation of transgenic rats using microinjection of plasmid DNA or lentiviral vectors / Severine Menoret [and others] -- Generation of transgenic animals by use of YACs / Almudena Fernandez, Diego Munoz, and Lluis Montoliu -- BAC transgenes, DNA purification, and transgenic mouse production / Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders -- Generation of transgenic animals with lentiviral vectors / Carlos Lois -- Vertebrate transgenesis by transposition / Aron Geurts, Darius Balciunas, and Lajos Mates -- Rat spermatogonial stem cell-mediated gene transfer / Karen M. Chapman [and others] -- Mouse cloning by nuclear transfer / Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama -- Gene targeting in embryonic stem cells / Elizabeth D. Hughes and Thomas L. Saunders -- Importance of mouse ES cell line selection / Wojtek Auerbach and Anna B. Auerbach -- Tetraploid complementation assay / Marina Gertsenstein -- Combining ES cells with embryos / Elizabeth Williams [and others] -- Derivation of murine ES cell lines / Kristina Nagy and Jennifer Nichols -- Rat embryonic stem cell derivation and propagation / Ping Li [and others] -- Induced pluripotency : generation of iPS cells from mouse embryonic fibroblasts / Han Li [and others] -- Preparation and analysis of DNA for use in transgenic technology / Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark -- Colony management / Karen Brennan -- Cryopreservation / B. Pintado and J. Hourcade -- Shipment of mice and embryos / Shirley Pease -- Pathogen-free mouse rederivation by IVF, natural mating and hysterectomy / J.M. Sztein, R.J. Kastenmayer, and K.A. Perdue -- Refinement, reduction, and replacement / Jan Parker-Thornburg |
| Summary |
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R's: refinement, reduction and replacement |
| Analysis |
genexpressieanalyse |
|
genomics |
|
diergenetica |
|
animal genetics |
|
transgenen |
|
transgenics |
|
Genetic Engineering |
|
Genetische modificatie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Animal genetic engineering.
|
|
Transgenic animals.
|
|
Animals, Genetically Modified
|
|
Gene Transfer Techniques
|
|
Animal genetic engineering
|
|
Transgenic animals
|
| Genre/Form |
Handbooks and manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Pease, Shirley.
|
|
Saunders, Thomas L
|
| ISBN |
9783642207921 |
|
3642207928 |
|
