| Description |
1 online resource (xiv, 407 pages) : illustrations |
| Series |
Springer protocols |
|
Springer protocols (Series)
|
| Contents |
Preparation of single-stranded DNA for pyrosequencing by LATE-PCR / Huiyong Yang [and others] -- Simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly / Yunlong Liu [and others] -- Improvement of LATE-PCR to prepare pyrosequencing template / Qinxin Song [and others] -- Pyrosequencing templates generated by nicking PCR products with nicking endonuclease / Qinxin Song [and others] -- Pyrosequencing templates generated by asymmetric nucleic acid sequence-based amplification (Asymmetric-NASBA) / Huning Jia [and others] -- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing / Pengfeng Xiao [and others] -- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing / Zhiyao Chen [and others] -- Novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit / Guohua Zhou [and others] -- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample / Guohua Zhou [and others] -- Gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactions directly from double-stranded PCR products / Guohua Zhou [and others] -- Improvement of pyrosequencing to allow multiplex SNP typing in a pyrogram / Guohua Zhou [and others] -- Improvement of pyrosequencing sensitivity by capturing free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase / Haiping Wu [and others] -- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing / Qinxin Song [and others] -- Preparation of thermal-stable biotinylated firefly luciferase and its application in pyrosequencing / Shuhui Zhu [and others] -- Expression of thermostable recombinant Luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system / Shu Xu [and others] -- Expression and purification of ATP sulfurylase from Saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing / Juan Luo [and others] -- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing / Jianping Wang [and others] -- Expression of PPDK from Microbispora rosea subsp. aerata in E. coli and its application in pyrosequencing / Bingjie Zou, Qinxin Song, and Guohua Zhou -- Construction of 3-plex barcodes for differential gene expression analysis with pyrosequencing / Xiaodan Zhang [and others] -- Development of pyrosequencing-based multiplex bioassay by designing barcodes encoded with artificially designed sequences / Zhiyao Chen [and others] -- Quantitatively discriminating multiplexed LAMP products with pyrosequencing-based bio-barcodes / Chao Liang [and others] -- Multiplex ligation-dependent sequence-tag amplification coupled with pyrosequencing for copy number variation (CNV) analysis / Haiping Wu [and others] -- Pyrosequencer miniaturized with capillaries to deliver deoxynucleotides / Guohua Zhou [and others] -- Pyrosequencing on acryl-modified glass chip / Huan Huang [and others] -- Pyrosequencing on-chip based on a gel-based solid-phase amplification / Huan Huang [and others] -- Prenatal diagnosis of trisomy 21 by quantitatively pyrosequencing heterozygotes using amniotic fluid as starting material of PCR / Hui Ye [and others] -- Comparative gene expression analysis of breast cancer-related genes by multiplex pyrosequencing coupled with sequence barcodes / Qinxin Song [and others] -- MicroRNA quantification by pyrosequencing with a sequence-tagged stem-loop RT primer / Hua Jing [and others] -- Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer / Qinxin Song [and others] -- Genotyping of pathogenic serotypes of S. suis with pyrosequencing / Huiyong Yang [and others] -- Differential gene expression analysis of breast cancer by combining sequence-tagged reverse-transcription PCR with pyrosequencing / Xiaodan Zhang [and others] -- Detection of avian influenza A virus by pyrosequencing / Weipeng Wang [and others] -- Genotyping of alcohol dehydrogenase gene by pyrosequencing coupled with improved LATE-PCR using human whole blood as starting material / Zheng Xia [and others] -- Pyrosequencing on a single cell / Qinxin Song [and others] |
| Summary |
Annotation Aiming to improve pyrosequencing protocols, as well as instrumentation for better clinical use, this volume describes improvements in and novel applications for pyrosequencing technology. Divided into five parts, it explores advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturisation of pyrosequencing equipment and various applications |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed February 10, 2016) |
| Subject |
Nucleotide sequence -- Methodology
|
|
Sequence Analysis, DNA -- methods
|
|
Base Sequence
|
|
Medical genetics.
|
|
Medical -- Genetics.
|
|
Medicine
|
|
Human genetics
|
|
Nucleotide sequence -- Methodology
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Zhou, Guohua
|
|
Song, Qinxin
|
| LC no. |
2015957401 |
| ISBN |
9781493933082 |
|
1493933086 |
|
149393306X |
|
9781493933068 |
|
